Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of microbial pre-fermentation coating multilayer microcapsule

A microbial fermentation and microcapsule technology, applied in the field of microbial technology and biology, can solve problems such as inappropriate microbial packaging, and achieve the effects of convenient reuse, reduced production cost, and good solubility

Active Publication Date: 2013-07-31
TIANJIN BIOFEED TECH CO LTD
View PDF10 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the method for coating microorganisms after fermentation in the prior art is not applicable to the problem of microbial coating treatment before fermentation, and then to provide a method for preparing microorganisms with high mechanical strength and good stability. layered microencapsulation method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of microbial pre-fermentation coating multilayer microcapsule
  • Preparation method of microbial pre-fermentation coating multilayer microcapsule
  • Preparation method of microbial pre-fermentation coating multilayer microcapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The yeast double-layer microcapsules described in this example are prepared through the following steps:

[0056] (1) Preparation of single-layer microcapsules: Under sterile conditions, take aseptically treated sodium alginate solution with a concentration of 1 g / L, add yeast seed solution in logarithmic growth phase to be coated, mix well, to 10 6 The density is about cfu / ml; and the mixed solution obtained above is filtered with a sterile filter membrane with a diameter of less than 0.22 μm, and the flow rate is 4m 3 / h of high-pressure air is extruded and sprayed into the aseptically treated calcium chloride solution with a concentration of 0.1mol / L through a hollow circular drop-shaped nozzle, stirred at a slow speed and solidified for 20 minutes, and the supernatant is filtered to obtain the required single-layer microcapsules, and washed with sterile water;

[0057] (2) Preparation of double-layer microcapsules: adding the single-layer microcapsules obtained in...

Embodiment 2

[0061] The yeast double-layer microcapsules described in this example are prepared through the following steps:

[0062] (1) Preparation of single-layer microcapsules: Under aseptic conditions, take aseptically treated sodium alginate solution with a concentration of 3 g / L, add the yeast seed solution to be coated, and mix evenly until 10 6 The density is about cfu / ml; and the mixed solution obtained above is filtered with a sterile filter membrane with a diameter of less than 0.22 μm, and the flow rate is 6m 3 / h of high-pressure air is sprayed into the aseptically treated calcium chloride solution with a concentration of 0.3mol / L through a hollow circular drop-shaped nozzle, stirred at a slow speed and solidified for 20 minutes, and the supernatant is filtered to obtain the required single Layer microcapsules, and wash with sterile water;

[0063] (2) Preparation of double-layer microcapsules: Add the single-layer microcapsules obtained in step (1) into an acidic buffer of ...

Embodiment 3

[0067] The yeast double-layer microcapsules described in this example are prepared through the following steps:

[0068] (1) Preparation of single-layer microcapsules: under aseptic conditions, take aseptically treated sodium alginate solution with a concentration of 1 g / L, add yeast seed liquid to be coated, and mix evenly; The mixed solution is filtered with a sterile filter membrane with a diameter of less than 0.22 μm and a flow rate of 4 m 3 / h of high-pressure air is sprayed into the aseptically treated calcium chloride solution with a concentration of 0.1mol / L through a hollow circular drop-shaped nozzle, stirred evenly and solidified, and the supernatant is filtered to obtain the required single-layer microcapsules , and washed with sterile water;

[0069] (2) Preparation of double-layer microcapsules: adding the single-layer microcapsules obtained in step (1) into an acidic buffer solution of chitosan with an initial concentration of 3g / L for film-forming reaction, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The present invention relates to a scale preparation method of microbial pre-fermentation coating multilayer microcapsule, and belongs to the fields of microbial technology and biotechnology. The method takes sodium alginate, calcium chloride and modified chitosan as the coated materials of microcapsule, wherein the deacetylation degree of the modified chitosan is more than 90% and molecular weight is about 40000; the method comprises the following steps of (1) preparing single-layer microcapsule and (2) preparing multilayer microcapsule. When performing second coating, a film of low concentration chitosan is formed, the amount of chitosan is little, and almost all of chitosan molecules can react with calcium alginate gel for polyelectrolyte complexation reaction, therefore, the compact film with good toughness can be easily formed; the film is thickened and the mechanical strength is increased along the increase of the chitosan concentration. Furthermore, the process is more convenient to reuse a buffer solution for dissolving chitosan, so as to reduce production costs.

Description

technical field [0001] The invention relates to a large-scale preparation method for coating multilayer microcapsules before microbial fermentation, and belongs to the field of microbial technology and biotechnology. Background technique [0002] Probiotics are live microbial preparations that can improve the host's ability to compete for intestinal mucosal receptors and enhance the body's immunity to exert their effects. Probiotics can produce β-galactosidase in the intestine, which can relieve the symptoms of lactose intolerance patients. Probiotics also have anti-tumor and anti-mutation effects, and can also improve the body's immunity. Therefore, the survival and proliferation ability of probiotics in the host has an important impact on the probiotic effect. The bacteria in probiotic products should have stable metabolism and strong activity. After passing through the upper digestive tract, a large number of them will survive and play a probiotic effect after entering t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23K1/00
Inventor 魏永刚杨禄良陈守慧张冬梅
Owner TIANJIN BIOFEED TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com