Method of detecting nucleotides of Bacillus cereus, primers for detecting and probes
A technology for detecting primers for Bacillus cereus, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve high sensitivity, high accuracy, and good primer specificity
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[0030] A method for detecting bacillus cereus, carried out according to the following steps:
[0031] 1. Design and synthesis of primers and probes
[0032] Download all Bacillus cereus genome sequences from the NCBI database, and select a highly conserved segment SEQ ID NO.4 with no secondary structure through comparative analysis of the sequences, and use the biological software PrimerExpress3.0 to design primers and Taqman probe. And the Blast homology comparison verification was carried out through the NCBI database to ensure that the sequence has no crossover with other species. The designed primer sequences are:
[0033] Forward primer: 5'-CATTCGTTCGCTGTGTGAACA-3'
[0034] Reverse primer: 5'-CCAACTTCAACCACAGGACAAA-3'
[0035] The sequence of the probe is: 5'-AGCCACCTAGCACACACGCCCAGA-3'
[0036] The 5' end of the probe was labeled with the reporter fluorescent dye VIC, and the 3' end was labeled with the quencher fluorescent dye BHQ2.
[0037] 2. Extraction of DNA ...
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