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Dasyatis akajei chondroprotein polypeptide capable of resisting prostate cancer, and preparation method and application thereof

An anti-prostate cancer and cartilage protein technology, applied in the field of anti-prostate cancer red ray cartilage protein polypeptides, can solve the problems of high-activity anti-tumor polypeptides that have not been reported, and achieve the effect of easy monitoring, scientific and reasonable process, and high activity

Inactive Publication Date: 2013-07-17
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the applicant found that the technology research on the preparation of anti-tumor polypeptide by enzymatic hydrolysis technology using red stingray cartilage as raw material is in a blank stage, while the enzymatic hydrolysis product is used as The preparation of highly active anti-tumor polypeptides and their applications has not been reported

Method used

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  • Dasyatis akajei chondroprotein polypeptide capable of resisting prostate cancer, and preparation method and application thereof
  • Dasyatis akajei chondroprotein polypeptide capable of resisting prostate cancer, and preparation method and application thereof
  • Dasyatis akajei chondroprotein polypeptide capable of resisting prostate cancer, and preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0036] 1) Take the washed and removed red stingray cartilage, and add the extract solution (containing 0.02 mol / L 2-N-morpholine ethanesulfonic acid and 0.02% EDT by mass volume fraction) according to the solid-liquid ratio of 1 g: 3~8 mL 1.0 mol / L guanidine hydrochloride solution), shaking and extracting at 20 °C for 72 h, and centrifuging at 9 000 r / min for 20 min at below 4 °C to obtain the crude extract of cartilage protein from red ray;

[0037]2) Add acetone to the crude extract of cartilage protein from the red ray until the concentration of acetone reaches 30%. After standing for 1 hour, centrifuge at 9 000 r / min for 20 minutes at below 4 ℃. Take the centrifuged supernatant and add acetone until the concentration of acetone reaches 30%. 60%, after standing for 1 hour, centrifuge at 9 000 r / min for 25 minutes at below 4 °C, take the precipitate and dialyze it in a dialysis bag with a molecular weight cut-off of 3 kDa for 24 hours, and freeze-dry the dialysate to obtain t...

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Abstract

The invention discloses a dasyatis akajei chondroprotein polypeptide capable of resisting a prostate cancer and a preparation method and application thereof. The amino acid sequence of the polypeptide is Ile-Glu-Pro-His (IEPH), and in ESI / MS detection, a molecular ion peak molecular weight of m / z 495.96 Da ([M+H]<+>) is given out. According to the invention, the preparation method provided by the invention is scientific and reasonable; the process of enzymatic hydrolysis can be easily monitored; and the prepared polypeptide has substantial inhibitory effects on proliferation of prostate cancer cells DU-145 and PC-3 and can be used for preparation of drugs for resisting the prostate cancer.

Description

[0001] technical field [0002] The invention relates to an anti-prostate cancer red ray cartilage protein polypeptide, and also relates to a preparation method of the anti-prostate cancer red ray cartilage protein polypeptide, and also relates to an application of the anti-prostate cancer red ray chondrocyte protein polypeptide. [0003] Background technique [0004] Peptide is a compound formed by linking α-amino acids with peptide chains, and it is also an intermediate product of proteolysis. Polypeptides are involved in the fields of hormones, nerves, immune regulation, cell growth and reproduction of the human body. They can regulate the physiological functions of various systems and cells in the body, activate relevant enzyme systems in the body, promote the permeability of intermediate metabolic membranes, or control DNA transcription. Or affect specific protein synthesis, and finally produce specific physiological effects. Existing studies have shown that pep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/103C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16C12P21/06A61K38/07A61P35/00
Inventor 王斌胡发远罗红宇徐银峰
Owner ZHEJIANG OCEAN UNIV
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