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Separation purification preparation process for activated monomers of celery seeds

A technology for separation and purification of active monomers, which is applied in the field of separation and purification of active monomers of celery seed, can solve the problems of time-consuming and laborious, few types of monomers, and the inability to meet the needs of pharmacological research and quality control, and achieve The effect of saving time, improving efficiency and optimizing process parameters

Inactive Publication Date: 2013-07-03
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction and separation methods of flavonoids mainly include Soxhlet extraction, microwave-assisted extraction, ultrasonic extraction, etc. The separation of monomers is mainly obtained by traditional silica gel column chromatography or preparative liquid chromatography. The traditional separation method consumes Time-consuming and labor-intensive, the types of monomers obtained in one experiment are small and the amount is small, which cannot meet the needs of pharmacological research and quality control

Method used

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  • Separation purification preparation process for activated monomers of celery seeds

Examples

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Comparison scheme
Effect test

Embodiment 1

[0023] The active monomer of separating and purifying celery seed comprises the following steps:

[0024] (1) Dry the celery seeds at a temperature of 30°C for 48 hours at a low temperature, and then crush them to 100 mesh;

[0025] (2) Extract the obtained dried celery seed powder with a supercritical extraction device, using supercritical carbon dioxide as the extraction agent, the purity of the extraction agent supercritical carbon dioxide used is 99.999%, at a pressure of 25MPa and a temperature of 45° C, the extraction agent flow rate is that extraction is carried out under the condition of 40L / h, and pressure is 6MPa, and temperature is to separate under the condition of 35 ℃, obtain the oily extract of celery seed;

[0026] (3) The oily extract of celery seed obtained in the previous step was separated and purified by high-speed countercurrent chromatography. The solvent system was petroleum ether-ethyl acetate-methanol-water, and the ratio was 5:5:7:3 (v / v), form imm...

Embodiment 2

[0029] The active monomer of separating and purifying celery seed comprises the following steps:

[0030] (1) Dry the celery seeds at a temperature of 25°C for 48 hours at a low temperature, and then crush them to 100 mesh;

[0031] (2) Extract the obtained dried celery seed powder with a supercritical extraction device, using supercritical carbon dioxide as the extraction agent, the purity of the extraction agent supercritical carbon dioxide used is 99.999%, at a pressure of 25MPa and a temperature of 40° C, the extraction agent flow rate is that extraction is carried out under the condition of 40L / h, and pressure is 6MPa, and temperature is to separate under the condition of 30 DEG C, obtain the oily extract of celery seed;

[0032] (3) The oily extract of celery seed obtained in the previous step was separated and purified by high-speed countercurrent chromatography. The solvent system was petroleum ether-ethyl acetate-methanol-water, and the ratio was 5:5:7:3 (v / v), form...

Embodiment 3

[0035] The active monomer of separating and purifying celery seed comprises the following steps:

[0036](1) Dry the celery seeds at a temperature of 35°C for 48 hours at a low temperature, and then crush them to 100 mesh;

[0037] (2) Extract the obtained dried celery seed powder with a supercritical extraction device, using supercritical carbon dioxide as the extraction agent, the purity of the extraction agent supercritical carbon dioxide used is 99.999%, at a pressure of 30MPa and a temperature of 50° C, the extraction agent flow rate is that extraction is carried out under the condition of 40L / h, and pressure is 6MPa, and temperature is to separate under the condition of 40 DEG C, obtains the oily extract of celery seed;

[0038] (3) The oily extract of celery seed obtained in the previous step was separated and purified by high-speed countercurrent chromatography. The solvent system was petroleum ether-ethyl acetate-methanol-water, and the ratio was 5:5:7:3 (v / v), form...

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Abstract

The invention discloses a separation purification preparation process for activated monomers of celery seeds, and relates to the field of separation and purification of the activated monomers of the celery seeds. According to the process, the extraction operation is carried out by a supercritical extraction device, and the separation and purification operations are carried out by a high-speed counter current chromatograph, so that the activated monomers of the celery seeds can be quickly and efficiently separated and purified in a high-purity way.

Description

technical field [0001] The invention relates to the field of separation and purification of active monomers of celery seeds, in particular to a preparation process for separation and purification of active monomers of celery seeds. Background technique [0002] Celery seed is the dry and ripe fruit of celery, which has the functions of dispersing gas, reducing swelling, relieving pain, diuresis and lowering blood pressure. At present, there are many studies on the volatile oil components contained in it, but there are few related studies on the flavonoids contained in it. Flavonoids have many physiological functions and medicinal value, and their main functions are: anti-aging, enhancing cardiovascular function, anti-inflammation, anti-allergy, antibacterial, anti-virus, anti-ulcer effect, hypoglycemic effect, anti-radiation and anti-inflammatory effect. Tumors, etc., but also have good application prospects in the development of food (and food additives), health care produ...

Claims

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Application Information

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IPC IPC(8): C07D307/88C07C69/587C07C67/48
CPCY02P20/54
Inventor 冯蕾冀海伟高琳琳李福荣崔英杰郭守东
Owner TAISHAN MEDICAL UNIV
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