Nutritional medium and culture method of cordyceps militaris

A nutrient matrix and Cordyceps militaris technology, which is applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems of low yield and easy variation of Cordyceps militaris, and achieve the goal of reducing bad variation, simple operation, and promoting market economy Effect

Inactive Publication Date: 2013-07-03
李向军
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Problems solved by technology

Because Cordyceps militaris has the above advantages, it has become the leader among the traditional Chinese medicine Cordyceps fungi of the genus Cordyceps, and various breeding methods have appeared in large numbers. However, because the female parent gene of Cordyceps militaris is easy to mutate, the yield has not been high.

Method used

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  • Nutritional medium and culture method of cordyceps militaris
  • Nutritional medium and culture method of cordyceps militaris

Examples

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example 1

[0012] 1) Prepare the culture medium under aseptic conditions. The nutrient matrix formula of Cordyceps militaris is: rice flour 40%, silkworm chrysalis powder 30%, malt powder 28%, peptone 1.5%; other additives are calcium nitrate 30mg / L, diphosphate Potassium hydrogen 20mg / L, magnesium sulfate 30mg / L and 2,4-dichlorophenylacetic acid 10mg / L.

[0013] 2) Cordyceps militaris breeding: After the culture medium is completed, select high-yield, high-quality, early-maturing fruiting bodies of Cordyceps militaris, and after 30 seconds of surface disinfection with 75% alcohol, clean the surface with sterile water, adjust the pH of the culture medium to 6.2, Inoculate it into the culture medium at ℃ and let it stand for culture, and treat it in the dark for a period of time. After the mycelium of Cordyceps militaris is covered with the medium, select high-quality fruiting bodies of Cordyceps militaris and perform another tissue separation to enter the stage of rapid propagation of Cor...

example 2

[0016] 1) Prepare the culture medium under aseptic conditions. The nutrient matrix formula of Cordyceps militaris is: rice flour 45%, silkworm chrysalis powder 35%, malt powder 18%, peptone 2%; other additives are calcium nitrate 45mg / L, diphosphate Potassium hydrogen 30mg / L, magnesium sulfate 50mg / L and 2,4-dichlorophenylacetic acid 15mg / L.

[0017] 2) Cordyceps militaris breeding: After the culture medium is completed, select high-yield, high-quality, early-maturing fruiting bodies of Cordyceps militaris, and after 30 seconds of surface disinfection with 75% alcohol, clean the surface with sterile water, adjust the pH of the culture medium to 6.8, and in 21 Inoculate it into the culture medium at ℃ and let it stand for culture, and treat it in the dark for a period of time. After the mycelium of Cordyceps militaris is covered with the medium, select high-quality fruiting bodies of Cordyceps militaris and perform another tissue separation to enter the stage of rapid propagatio...

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Abstract

The invention particularly relates to a nutritional medium and a culture method of cordyceps militaris. The nutritional medium is prepared by taking rice meal and silkworm chrysalis meal as main materials, and added with calcium nitride, magnesium sulfate, 2,4-dichlorophenylacetic acid and other substances, so as to reduce bad heteromorphosis during the reproductive process of the cordyceps militaris, and accelerate the growth rate. The culture method is simple to operate, but can not pollute the environment. The obtained cordyceps militaris is high in yield and good in quality, can be produced by industrialization to promote market economy.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a nutrient substrate and a cultivation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris belongs to the genus Cordyceps ergotaceae, also known as Cordyceps militaris, Cordyceps militaris, and Cordyceps militaris. The sub-seats are solitary or several grow together from the head or nodes of the parasitic pupal body, the color is orange or orange red, and the total length is 2-8 cm. The color of the pupal body is purple, and the length is about 1.5-2 cm. Worldwide distribution of natural resources is very small. In winter, the larvae hibernate in the soil, where the fungi parasitize and absorb nutrients, and the larvae are filled with mycelia and die. In summer, seedlings grow from the corpses of the larvae, which resemble grass and are collected around the summer solstice. Traditional Chinese medicine believes that Co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
Inventor 李向军
Owner 李向军
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