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Preparation method of functional oral preparation rich in erythrothioneine

A technology for ergothioneine and oral preparations, which is applied in the field of preparation of functional oral preparations, can solve the problems of high cost and difficulty in chemical synthesis, and achieve the effects of high utilization rate, high safety, and low energy consumption

Active Publication Date: 2013-07-03
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Ergothioneine can be synthesized chemically, but because it is a chiral compound, chemical synthesis is extremely difficult and costly

Method used

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  • Preparation method of functional oral preparation rich in erythrothioneine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The acquisition of the edible fungus mycelia rich in ergothioneine comprises the following steps:

[0024] (1) Culture medium preparation: take by weight 30 parts of dextrin, 15 parts of casein, 3 parts of K 2 SO 4 , 2 parts (NH 4 )H 2 PO 4 and 1.5 parts of MgCl 2 , add water to 1000 parts, adjust pH=5.5;

[0025] (2) Shake flask culture: put 150mL medium in a 500mL Erlenmeyer flask, sterilize at 121°C for 20min; inoculate each Erlenmeyer flask with 1cm 2 , under the conditions of 25°C and 150rpm, shaking culture in a shaking table for 9 days, a large amount of ergothioneine was synthesized and accumulated in the mycelia of the mushroom;

[0026] (3) Obtain the edible fungus mycelia rich in ergothioneine by solid-liquid separation of the fermented liquid.

Embodiment 2

[0028] The acquisition of the edible fungus mycelia rich in ergothioneine comprises the following steps:

[0029] (1) Culture medium preparation: take by weight 40 parts of glycerol, 15 parts of soybean meal powder, 3 parts of K 2 SO 4 , 3 parts (NH 4 )H 2 PO 4 and 1.5 parts of MgCl 2 , add water to 1000 parts, adjust pH=5.8;

[0030] (2) Shake flask culture: put 150mL medium in a 500mL Erlenmeyer flask, and sterilize at 121°C for 30min; inoculate each Erlenmeyer flask with 2cm 2 , under the conditions of 26°C and 140rpm, shaking culture on a shaker for 10 days, a large amount of ergothioneine was synthesized and accumulated in the mycelia of Pleurotus wild adenophora.

[0031] (3) Obtain the edible fungus mycelia rich in ergothioneine by solid-liquid separation of the fermented liquid.

Embodiment 3

[0033] The preparation method of the functional oral liquid rich in ergothioneine, comprises the steps:

[0034] (1) 1 part of the edible fungus mycelia rich in ergothioneine prepared in Example 1 was mixed with 8 parts of water, stirred and leached at 100 rpm for 1 hour at 80° C.; concentrated for 0.01 hour under the condition of 0.01 MPa, Prepare concentrated solution, the ergothioneine content in the concentrated solution is 1mg / mL;

[0035] (2) Measure the following raw materials: 600mL of concentrated solution obtained in step (1), 0.2g of xanthan gum (stabilizer), 0.3g of phospholipid, 0.4g of aspartame (flavoring agent), 20g of xylitol, essence 5g, 400mL purified water;

[0036] (3) After fully mixing xanthan gum, phospholipids, aspartame and xylitol, dissolve it with a small amount of water, then add the concentrated solution and the remaining water, and pass through the colloid mill twice to make it evenly mixed add essence;

[0037] (4) The material obtained in st...

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Abstract

The invention discloses a preparation method of a functional oral preparation rich in erythrothioneine, which comprises the following steps: firstly, edible fungus mycelium rich in erythrothioneine is mixed with water, and under the temperature of 80 to 100 DEG C, the mixture is stirred and leached; secondly, a concentrated solution is obtained through concentration, and acceptable additives of food are added to the concentrated solution, so that the liquid oral preparation is obtained. The edible fungus mycelium rich in the erythrothioneine obtained by adopting a submerged fermentation biosynthesis method of the edible fungus mycelium is used as a raw material; and after treated by the process steps of the method, the obtained functional oral preparation, prepared from the concentrated solution or a dried material of the concentrated solution is high in product safety. The process provided by the invention has the advantages that all elements of the edible fungus mycelium rich in the erythrothioneine are comprehensively utilized; yield loss caused by extraction of functional elements through purification process is zero; the steps of the process are simple; the energy consumption is less; the erythrothioneine in the body of the mycelium is leached out of cells; and the usage efficiency of the erythrothioneine is high.

Description

technical field [0001] The invention relates to a preparation method of an oral preparation, in particular to a preparation method of a functional oral preparation rich in ergothioneine. Background technique [0002] Ergothioneine (L-Ergothioneine) is a rare natural amino acid strong antioxidant that exists in most animals and plants. In the human body, it can maintain system oxidation-reduction balance, detoxify foreign substances, resist oxidative damage, and prevent radiation sexual injury and cancer prevention. The chemical structural formula of ergothioneine is as follows: [0003] [0004] Ergothioneine, whose chemical name is 2-mercaptohistidine trimethyl inner salt, was first discovered in ergot bacteria (Claviceps purpurea) in 1909 (TANRET C., Sur une base nouvelle retiree du seigle ergote, L-Ergothioneine Compt.Rend.Acad.Sci., 1909.149:222-224), is the only natural 2-thioimidazole amino acid (2-thio-imidazole) known so far. The human body contains ergothionei...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61K31/4172A23L1/29A61P35/00A61P39/02A61P39/06A23L33/00
Inventor 姜文侠杨萍刘琦
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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