Method for preparing oil in auxiliary cold pressing manner by solid-state fermentation of oil crops by two steps via aspergillus oryzae

Abstract

The invention relates to a process for extracting oil from oil crops such as soybeans in a cold pressing manner by performing the solid-state fermentation in two steps via aspergillus oryzae. The process comprises the following steps: peeling, smashing and soaking the oil crops such as the soybeans; performing sterilization or microwave sterilization on the oil crops in a high-handed sterilization pan; regulating the water content; grafting a bacterial logarithmic phase seed solution and a spore suspension solution into a solid substrate; performing oil dissociation in two stages, wherein cultivating enzymes generated by the solid-state fermentation and measuring the enzyme activity in the first stage and releasing oil ingredients by performing the solid-state enzymolysis in the second stage; performing vacuum drying on the fermented material; putting the fermented material into a cool pressing machine; and extracting the oil in the cold pressing manner. By virtue of the growth of aspergillus oryzae mycelial bodies, the bacterial enzyme systems can permeate and act on the histocytes of the oil crops such as the soybeans and the like so as to damage the cellulose skeletons of the histocytes, so that lipoprotein complexes are completed disintegrated, and therefore, the organization structures of the oil crops are collapsed. As a result, the grease is released fully, i.e., the oil extraction rate of the oil crops in the cold pressing manner is improved greatly. The method has the characteristics of high raw material oil extraction rate, no residual organic solvent, safe oil quality and the like, and is convenient to operate.

Description

technical field [0001] The invention relates to a method for extracting vegetable oil, in particular to a method for extracting oil from soybeans and other oil crops by cold pressing after solid-state fermentation and enzymolysis by microorganism Aspergillus oryzae. Background technique [0002] The current conventional industrial oil production methods continue to use the traditional hot pressing process and organic solvent extraction. Although these two oil extraction processes have high oil extraction efficiency, they all have their own disadvantages. Steaming and frying are used in hot pressing, which leads to the maximum denaturation of high-quality protein, wastes a lot of protein resources, and indirectly increases the production cost. Although the oil extraction rate of organic solvent extraction is high, the chemical solvent used for oil extraction will A small amount remains in the refined oil and cake, and long-term consumption may cause certain harm to human heal...

AI Technical Summary

Problems solved by technology

At present, there are relatively few studies on the oil extraction technology combined with solid-state fermentation and cold pressing, especially the research on the influence of different strains on this process needs to be further explored and improved. Finding the appropriate strain is the key to affect the oil extraction effect and the quality of by-products

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