Cell biological method for predicting body height of excellent ice-snow athlete by utilizing single nucleotide polymorphism of promoter region of GH1 gene

A cell biology and athlete technology, applied in the interdisciplinary field of sports science and life science, can solve the problems that are not established in life science, and outstanding snow and ice athletes start late, and achieve the effect of simple and easy operation.

Inactive Publication Date: 2013-06-19
HARBIN INST OF PHYSICAL EDUCATION
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Problems solved by technology

[0007] Since the research on the optimal height of outstanding ice and snow athletes started relatively late in our country, and most of the training and selection of ice and snow athletes are based on experience, not on the basis of life science

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0012] The peripheral blood of ordinary people and elite ice and snow athletes were selected as the control group and the experimental group, respectively.

[0013] (1) Genomic DNA extraction: Genomic DNA was extracted from peripheral blood by the phenol-chloroform method, and stored at -20°C for future use.

[0014] (2) PCR amplification of the region adjacent to the GH1 gene promoter: using touchdown PCR, the reaction parameters are: 94°C, 5min; 94°C, 30s; The annealing temperature of each cycle is 59°C), 72°C for 1min, and cycled 25 times; 72°C for 7min. PCR products were observed by 1.5% LMA electrophoresis.

[0015] (3) Purification, cloning and sequencing of PCR products: Purification and cloning of the products were carried out according to the instructions in the kit, and sequencing was performed after cloning.

[0016] (4) SNP detection and analysis: two SNP sites were found, namely S3:G-308T and S7:T-57G.

[0017] (5) The polymorphic changes of these two loci affe...

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Abstract

The invention relates to a method for predicting the body height of an excellent ice-snow athlete on the level of cell biology. The method is based on the analysis of the relation between single nucleotide polymorphism (SNP) in a promoter proximal region of a GH1 gene and regulation and control of the body height of a human body, and the relation between the SNP and the body height. The characteristics of the body heights of the existing excellent ice-snow athletes around the world can be investigated by utilizing the method, thereby having a certain reference value for selecting and training talents for the item in China.

Description

technical field [0001] The invention relates to a cell biology method for predicting the height of an excellent ice and snow athlete. It is suitable for the early selection of excellent ice and snow athletes at the gene and protein levels, and belongs to the interdisciplinary field of sports science and life science. Background technique [0002] Human height is a complex quantitative trait. The final height of ice and snow athletes is determined by the interaction of genetic, environmental, psychological, disease and other factors. Studies have shown that the proportion of normal people's height and body weight affected by genetic factors is 37% to 53%. For this complex quantitative trait, apart from single gene mutations with a low incidence, single nucleotide polymorphisms (singlenucleotide polymorphism, SNP) on some associated genes are the genetic basis for human height differences. [0003] The linear growth of ice and snow athletes depends on the stability of the g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 郭俊清刘义峰谢长福王文杰单志强关伟军
Owner HARBIN INST OF PHYSICAL EDUCATION
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