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Method for acquiring anti-sheath blight gene associated resistance gene analogs and molecular markers thereof

A technology of disease resistance homologous sequence and acquisition method, which is applied in the field of sheath blight resistance gene association disease resistance homologous sequence and molecular marker acquisition field, can solve the problem of low value of breeding and utilization of sheath blight resistance, and reduce sheath blight. disease hazards, simplifying breeding programs, and improving the effect of resistance

Inactive Publication Date: 2013-06-12
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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Problems solved by technology

However, since sheath blight resistance is a quantitative trait, and the location of QTLs for sheath blight resistance mostly uses molecular markers with non-gene functions, such as microsatellite markers (SSR), these molecular markers are of great importance in breeding for sheath blight resistance. Use value is not high

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  • Method for acquiring anti-sheath blight gene associated resistance gene analogs and molecular markers thereof
  • Method for acquiring anti-sheath blight gene associated resistance gene analogs and molecular markers thereof
  • Method for acquiring anti-sheath blight gene associated resistance gene analogs and molecular markers thereof

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Embodiment

[0074] Example: RGA genotype association analysis of sheath blight resistance phenotype

[0075] 1. Phenotypic association analysis of molecular resistance to sheath blight

[0076] A total of 472 pairs of RGA primers were designed through the genome-wide resistance gene analog sequence (RGA), and 472 pairs of RGA primers were used in 137 US Department of Agriculture core rice germplasms (cited from the Rice Research Center of the US Department of Agriculture Service) A total of 719 PCR fragments were amplified, and 138 pairs of RGA primers amplified polymorphisms in 137 core collections.

[0077] From 2009 to 2011, the sheath blight resistance of 37 core collections was identified for three consecutive years through the microinjection method of sheath blight bacteria, and the sheath blight resistance phenotypes of 137 core collections were obtained.

[0078] Combining the RGA genotype and the sheath blight resistance phenotype, using a single molecular marker analysis method...

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Abstract

The invention discloses a method for acquiring anti-sheath blight gene associated resistance gene analogs of rice and molecular markers thereof. The method comprises: designing RGA (resistance gene analog) primers for complete genome resistance analogs to obtain genotypes of rice germplasms, acquiring anti-sheath blight phenotypes of 178 core germplasms through a sheath blight pathogen microtubule identification technology, combining the RGA genotypes and the anti-sheath blight phenotypes to carry out molecular character association analysis and performing clone sequencing on a PCR product, thus obtaining the anti-sheath blight associated resistance gene analogs and molecular markers thereof. In the invention, by means of genetic transformation and hybridization, the anti-sheath blight gene associated resistance gene analogs can be introduced into common cultivated rice to improve the resistance of rice to sheath blight. The anti-sheath blight gene associated molecular markers obtained in the invention can be used for detecting the existence of anti-sheath blight genes, realizes indirect selection of disease-resistant plants, and can exempt identification of inoculated sheath blight pathogens from the influence of climate, geographical positions and other uncertain factors, thus being able to be used for early generation selection, anti-sheath blight variety breeding period shortening, and breeding efficiency improvement.

Description

technical field [0001] The invention relates to the field of plant genetic engineering of plant disease-resistant genes and molecular marker-assisted breeding, in particular to the homologous sequence of the disease-resistant gene of the sheath blight resistance gene and its molecular marker, and the application of special primers thereof in rice sheath blight resistance . Background technique [0002] Rice sheath blight is a fungal disease caused by the infection of Rhizoctonia solani Kühn, which occurs to varying degrees in various rice regions around the world. The disease can occur during the entire growth period of rice, and generally results in a 10-30% reduction in rice yield, and can reach 50% in severe cases. In recent years, due to the promotion of high-yielding varieties, the increase of planting density, and the increase of nitrogen fertilizer application, the damage of rice sheath blight has become increasingly serious, and it has become the first major disease...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10
Inventor 任鄄胜曾礼华任光俊高方远陆贤军吴贤婷
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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