Application of Lactobacillus casei KL1 for producing bile salt hydrolase in functional sour milk

Abstract

The invention relates to an application of Lactobacillus casei KL1 for producing bile salt hydrolase in functional sour milk, and is suitable for assimilate cholesterol functional sour milk and microecology preparation production in fermented dairy product. The application provided by the invention employs a straight casting mode to add Lactobacillus casei KL1 (CGMCC No.1809) dry powder leavening agent used for effectively reducing cholesterol and a common sour milk dry powder leavening agent to prepare the functional sour milk with excellent quality. On a base that a common sour milk fermentation technology is not changed, the characteristic of KL1 bacterial strain for producing bile salt hydrolase can be employed to prepare the sour milk for reducing serum cholesterol, the sour milk whey precipitation problem can be solved, the post-acidifying degree of the sour milk can be reduced, the sour milk viscosity is enhanced, the product is stored for 21 days at the temperature of 4 DEG C, the live bacteria quantity of Lactobacillus casei KL1 can reach more than 106CFU / g, and the Lactobacillus casei KL1 has health care function. The prepared functional sour milk has simple fermentation technology, short fermentation period and low cost, and is adapted to large scale industrial production.

Description

technical field [0001] The invention relates to a functional yogurt production technology for mixed fermentation of Bile Salt Hydrolase (BSH) Lactobacillus casei (Lactobacillus casei) KL1 dry powder starter and ordinary yogurt dry powder starter, which is suitable for lowering cholesterol in fermented dairy products Production of functional yogurt and probiotics. Background technique [0002] Probiotics refer to a class of microorganisms that improve the microecological balance of specific parts of the host through intestinal colonization and have several other beneficial physiological functions. They can improve the ecological balance of intestinal microorganisms, control intestinal infection, reduce serum cholesterol levels, improve the utilization of lactose for people with lactose intolerance, and can stimulate specific or non-specific immune responses, and have the effect of immune enhancement. The live bacteria of probiotic products should have stable metabolism and s...

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Problems solved by technology

[0006] Although there is also a patent report about the research on Lactobacillus casei lowering serum cholesterol effect abroad: Lactobacillus casei bd-II stain and used to reduce blood cholesterol (20060127380), but at present, the content of bile salt hydrolyzing enzyme produced by Lactobacillus casei abroad is still unknown. No patent report

Although there is a patent report on "Functional Yogurt Containing Lactobacillus reuteri and Its Preparation Method (200310112715.8)" in China, there are domestic reports on the application of Lactobacillus casei to the production of functional yogurt using cholesterol-lowering and high-yielding bile salt hydrolyzing enzymes. Have not yet seen relevant literature reports and patent reports

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