Wettable powder of streptomyces roseoflavus biological bactericide

Abstract

The invention discloses wettable powder of a streptomyces roseoflavus biological bactericide, which adopts diatomite as a matrix and comprises the following components: not less than 108 propagules of streptomyces roseoflavus strain 0116, and 4% of a wetting dispersant. It is determined that diatomite is used as a carrier and sodium lignosulphonate is used as the wetting dispersant. In addition, the strain wettable powder storage stability is determined by regular detection of the viable bacterial count, and results show that after strain wettable powder storage for 24 months, the viable bacterial count is more than 80%. The invention has the characteristics of short development period, low cost, simple process, and capability of being developed into various dosage forms, and is suitable for large-scale production. Since propagules of streptomyces roseoflavus strain 0116 is used as the main component of the wettable powder, the invention is suitable for large-scale production, and when compared with strain fermentation liquor, the invention greatly shortens the field using amount, and reduces packaging and transport cost.

Description

Technical field: [0001] The invention relates to a diatomite-based Streptomyces roseus flavum biological fungicide wettable powder for preventing and controlling plant diseases. Background technique [0002] At present, the prevention and control of plant diseases is mainly based on chemical pesticides. Their long-term use has caused great selection pressure on pathogenic bacteria and easy to produce drug resistance. Moreover, the high residue and high pollution of chemical pesticides have a serious impact on the environment and the safety of people and animals. Therefore, bio-pesticides with low toxicity and low residue have become a major trend in the development and production of pesticides. Hebei Agricultural College Students' Defense Office applied for two patents on biocontrol preparations on November 2, 2005 and June 9, 2009, namely "a biological fungicide and its preparation method, application number / patent number: 200510117421" And "a biological fungicide wettabl...

AI Technical Summary

Problems solved by technology

However, the defects of the above two patent applications are: in the previous patent, the use of microbial secondary metabolites as pesticides or their lead compounds requires the exact structure of the secondary metabolites to be clarified

The secondary metabolites of the strain play a major role in the fermentation broth of the 0116 strain, and the cost and cycle of separating, purifying and identifying the components of the fermentation broth are relatively high and the cycle is long, so chemical synthesis cannot be carried out, nor can it mass production

At the same time, since the main component of the fermentation liquid is water, the unit consumption of the fermentation liquid directly applied to the field is large, resulting in high packaging and transportation costs.

The defect of the former patent has been improved in the patent of "a biological fungicide wettable powder", but the latter patent has more complicated procedures in the specific implementation process, including first cultivating liquid seeds, and then transferring them to corn stalks. Solid fermentation, after freeze-drying, collecting pure spore powder, then mixing certain carrier and wetting and dispersing agent, finally making wettable powder

In this way, the whole process takes a long time and the steps are cumbersome, and the process of freeze-drying and collecting pure spore powder is complicated in large-scale production, and the cost is high, especially the collection of pure spore powder, which is difficult for mass production.

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