Heart-type fatty acid binding protein content detection kit and preparation method thereof
A fatty acid combination and detection kit technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of long time-consuming, cumbersome operation and low sensitivity of enzyme-linked immunoassay detection, and is conducive to large-scale promotion and application and detection operation The effect of simplicity and high measurement accuracy
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Embodiment 1
[0043] Preliminary Example 1 Preparation of Latex Particles Coated with Heart-Type Fatty Acid Binding Protein Antibody
[0044] (1) Activated latex: Take 1 mL of polystyrene latex solution (purchased from PolyMicrospheres) with a concentration of 10% and a particle size of 90 nm in a 50 mL centrifuge tube, then add 9 mL of 0.12M phosphate buffer solution to the centrifuge tube, and mix well , to obtain a diluted latex solution; accurately weigh 50 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and dissolve it in 1 mL of 0.12M phosphate buffer solution, and add this solution to complete the dilution In the latex solution, oscillate and mix evenly, and place on a 25°C constant temperature shaker, 200 rpm, and react for 1h; On a shaker, 200 rpm, react for 10 minutes;
[0045] (2) Sensitized latex: Add 2 mL of 1 mg / mL heart-type fatty acid binding protein polyclonal antibody (purchased from Wuhan Huamei Bioengineering Co., Ltd.) to the solution completed in step (1), ...
Embodiment 2
[0048] Preliminary Example 2 Preparation of latex particles coated with heart-type fatty acid binding protein antibody
[0049] (1) Activated latex: Take 1 mL of polystyrene latex solution with a concentration of 10% and a particle size of 200 nm (purchased from PolyMicrospheres) in a 50 mL centrifuge tube, then add 9 mL of 0.12M phosphate buffer solution to the centrifuge tube, and mix well , to dilute the latex solution. Accurately weigh 50mg of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and dissolve it in 1mL of 0.12M phosphate buffer solution, and add this solution into the diluted latex solution, shake Mix evenly, and place on a constant temperature shaker at 25°C, 200 rpm, and react for 1 hour; after the reaction is completed, add 2 μL of 2-mercaptoethanol to the above system, shake and mix evenly, and place on a constant temperature shaker at 25°C, 200 rpm min, reaction 10min;
[0050] (2) Sensitized latex: Add 2 mL of 1 mg / mL heart-type fatty acid binding pr...
Embodiment 3
[0053] Preliminary Example 3 Preparation of Latex Particles Coated with Heart-type Fatty Acid Binding Protein Antibody
[0054] (1) Activated latex: Take 1 mL of polystyrene latex solution with a concentration of 10% and a particle size of 120 nm (purchased from PolyMicrospheres) in a 50 mL centrifuge tube, then add 9 mL of 0.12M phosphate buffer solution into the centrifuge tube, and mix well , to dilute the latex solution. Accurately weigh 50mg of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and dissolve it in 1mL of 0.12M phosphate buffer solution, and add this solution into the diluted latex solution, shake Mix evenly, and place on a constant temperature shaker at 25°C, 200 rpm, and react for 1 hour; after the reaction is completed, add 2 μL of 2-mercaptoethanol to the above system, shake and mix evenly, and place on a constant temperature shaker at 25°C, 200 rpm min, reaction 10min;
[0055] (2) Sensitized latex: Add 2 mL of 1 mg / mL heart-type fatty acid binding ...
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