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Method for RNA (ribonucleic acid) interference of panonychus citri mites

A citrus pan-clawed mite and RNA interference technology, applied in the field of citrus, can solve the problems of serious drug resistance, affecting fruit safety, restricting the use period, scope and control effect of pesticides, achieving broad market prospects, reducing the amount of use, and designing novel effect

Inactive Publication Date: 2013-04-24
CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical control is still an important measure to control Panonychus citrus. Improper application of chemical pesticides will not only affect the safety of fruits, but also lead to serious drug resistance. The resistance of harmful mite directly restricts the service life, scope and control effect of pesticides.
RNA interference (RNA interference, RNAi) is a very promising new method of pest control, which can provide a new way to better control the pests that affect the yield of food and economic crops in production. This technology is the traditional pest control technology. A new breakthrough with broad application prospects. At present, many important pest-specific RNA interference technologies have been constructed, but the RNA interference technology for Panonychus citrus, especially its nymphs, has not yet been established.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1), the rearing of citrus panonychia nymphs

[0019] Wash and dry the petri dish with a diameter of 9 cm, spread a 0.7 cm thick sponge on the petri dish, spread filter paper with a diameter slightly smaller than the sponge on the petri dish, add water until it is level with the filter paper, and make a stepped water isolation platform. Pick the complete and vigorously growing citrus leaves, soak them in water and wash them, put the leaves face down on the filter paper of the stepped water isolation platform, and press the leaf edge with wet absorbent cotton. Inoculate the female adult mites of Panonychus citrus on the leaves, remove the female adults after 24 hours of laying eggs, and place the eggs in a constant temperature light incubator, control the temperature at 25°C, relative humidity at 70%-80%, and photoperiod L : D=(14:10)h, that is, long light: short light=(14:10)h, continuous culture for 5 days.

[0020] (2), carry out dsRNA solution processing to citrus p...

Embodiment 2

[0026] (1), the rearing of citrus panonychia nymphs

[0027] Wash and dry the petri dish with a diameter of 9 cm, spread a 0.7 cm thick sponge on the petri dish, spread filter paper with a diameter slightly smaller than the sponge on the petri dish, add water until it is level with the filter paper, and make a stepped water isolation platform. Pick the complete and vigorously growing citrus leaves, soak them in water and wash them, put the leaves face down on the filter paper of the stepped water isolation platform, and press the leaf edge with wet absorbent cotton. Inoculate female adults of Panonychus citrus on the leaves, remove the female adults after 24 hours of laying eggs, place the eggs in a constant temperature light incubator, control the temperature at 26°C, relative humidity at 70%-80%, and photoperiod L : D = (14:10) h continuous culture for 7 days.

[0028] (2), carry out dsRNA solution processing to citrus panonychia nymph

[0029] Pick nymphs on new leaves, d...

Embodiment 3

[0034] (1), the rearing of citrus panonychia nymphs

[0035] Wash and dry the petri dish with a diameter of 9 cm, spread a 0.7 cm thick sponge on the petri dish, spread filter paper with a diameter slightly smaller than the sponge on the petri dish, add water until it is level with the filter paper, and make a stepped water isolation platform. Pick the complete and vigorously growing citrus leaves, soak them in water and wash them, put the leaves face down on the filter paper of the stepped water isolation platform, and press the leaf edge with wet absorbent cotton. Inoculate female adults of Panonychus citrus on the leaves, remove the female adults after 24 hours of laying eggs, place the eggs in a constant temperature light incubator, control the temperature at 24°C, relative humidity at 70%-80%, and photoperiod L : D = (14:10) h continuous culture for 6 days.

[0036] (2), carry out dsRNA solution processing to citrus panonychia nymph

[0037]Pick the hatched nymphs on ne...

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PUM

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Abstract

The invention discloses a method for RNA (ribonucleic acid) interference of panonychus citri mites. The method includes the steps of feeding the panonychus citri mites, treating the panonychus citri mites with dsRNA solution, determining the molting and survival situation of treated the panonychus citri mites, and evaluating the effect of the RNA interference. The method has novel and reasonable design, enables the panonychus citri mites not to complete the normal molting so as to die with RNA interference, and thus, the purpose of preventing the panonychus citri mites is achieved, the amount of the chemical pesticides to be used can be reduced, and accordingly the method has a broad application prospect.

Description

technical field [0001] The invention relates to the field of citrus, in particular to a method for RNA interference of Panonychus citrus nymphs. Background technique [0002] Panonychus citrus is a worldwide pest of citrus, which has a great impact on the yield and quality of citrus. At present, chemical control is still an important measure to control Panonychus citrus. Improper application of chemical pesticides will not only affect the safety of fruits, but also lead to serious drug resistance. The resistance of harmful mite directly restricts the service life, scope and control effect of pesticides. . RNA interference (RNA interference, RNAi) is a very promising new method of pest control, which can provide a new way to better control the pests that affect the yield of food and economic crops in production. This technology is the traditional pest control technology. The new breakthrough has broad application prospects. At present, many important pest-specific RNA inter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 冉春陈飞刘浩强李鸿筠张云飞
Owner CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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