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Method for in-situ remediation of arsenic-polluted soil by arsenic-resistant bacteria with induction and solidification effects

An in-situ remediation and arsenic pollution technology, applied in the field of biological environmental pollution remediation, can solve the problems of difficult application of bioremediation technology, increase the difficulty of bioremediation, etc. Effect

Inactive Publication Date: 2013-05-29
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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Problems solved by technology

However, because the bacteria adsorbed by the arsenic cannot be separated, they still exist in the environment, and then due to various reasons, the arsenic that has been absorbed by the bacteria will be released into the environment again
In addition, the sensitivity of most microorganisms to redox conditions and changes in the valence state of toxic metals has led to some difficulties in the practical application of bioremediation technology.
In addition, some high salinity soils make bioremediation more difficult

Method used

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  • Method for in-situ remediation of arsenic-polluted soil by arsenic-resistant bacteria with induction and solidification effects

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Embodiment

[0024]a. The soil in the area with serious arsenic pollution is selected as the arsenic-contaminated soil in a high-salt environment. The arsenic-polluted environment is that the heavy metals in the soil are in an exchangeable state and combined with carbonate, including arsenic-contaminated soil, cesium-contaminated soil, and copper-contaminated soil Soil, lead-contaminated soil, put in a sterilized bottle with a cover, put 1-2g of soil sample in a conical flask containing 50ml of nutrient broth, the pH of the nutrient broth is 8-8.5, and the arsenic content is 100mg L -1 , and then place the Erlenmeyer flask at a speed of 130rpmmin -1 , cultivated for 48 hours at a temperature of 30°C to obtain a bacterial solution;

[0025] b. Add the cultured bacterial solution to the arsenic content of 100mg L respectively -1 , 250mgL -1 , 500mg L -1 The nutrient broth medium is peptone 10g L -1 , beef extract 1.5g L -1 , yeast powder 1.5g L -1 and sodium chloride 5g L -1 Among the...

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Abstract

The invention relates to a method for in-situ remediation of arsenic-polluted soil by arsenic-resistant bacteria with induction and solidification effects. The method comprises the steps of: firstly, separating arsenic-resistant bacteria which can generate more urea decomposition enzymes under an environment of resisting high-concentration arsenic by gradually increasing the arsenic content in a nutrient bouillon culture medium; putting the arsenic-resistant bacteria in the nutrient bouillon culture medium containing urease and CaC12; gathering to obtain the arsenic-resistant bacteria with calcification; mixing the gathered arsenic-resistant bacteria CR5 bacteria solution with calcification with the polluted soil containing arsenic to form a mixed solution, so that the pH of the mixed solution displays a weak alkaline environment until the pH is 8-9; cultivating the mixed solution in a wet environment; and fixing the arsenic in the polluted soil containing arsenic by the arsenic-resistant bacteria with calcification by inducing calcification, so that the arsenic-polluted soil is subjected to in-situ remediation. The method is simple and convenient to operate, easy to control, and low in cost, and can be widely applied to repairing of heavy metal soil pollution.

Description

technical field [0001] The invention relates to a method for in-situ restoration of arsenic-contaminated soil by arsenic-resistant bacteria using induced solidification, and belongs to the technical field of bio-environmental pollution restoration. Background technique [0002] Arsenic is a highly toxic element, and since its discovery in 1250, there have been many serious cases of arsenic poisoning. At least 50 million people around the world are facing the threat of endemic arsenic poisoning. Among them, the arsenic pollution in Asia is the most serious, mainly concentrated in Bangladesh, India and China. Arsenic pollution in soil and water environment has become an environmental problem that has attracted worldwide attention. Arsenic is usually 3+ and As 5+ form in the soil. Arsenic compounds are extremely toxic. The poisoning dose of arsenic to humans is 0.01-0.052 grams, and the lethal dose is 0.06-0.2 grams. Studies have found that high concentrations of arsenic ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 潘响亮宋文娟张道勇高磊
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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