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MiRNA marker associated with post-esophageal-cancer-operation early recurrence and prognosis and applications thereof

A marker, esophageal squamous cell carcinoma technology, applied in the field of genetic engineering and oncology, can solve the problems of lack of specific and sensitive molecular markers, not identical molecular biological changes, lack of early recurrence and metastasis of esophageal cancer, etc. To achieve the effect of improving sensitivity and specificity, simple method and convenient operation

Active Publication Date: 2015-03-25
河南普诺易生物制品研究院有限公司 +1
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AI Technical Summary

Problems solved by technology

[0004] At present, there are many studies and reports on tumor molecular markers of esophageal cancer, except for the traditional tumor markers carcinoembryonic antigen (CEA), squamous cell carcinoma-related antigen (SCC), cytokeratin fragment 19 (CYFRA2l–l ), p53 protein antibody (p53-Ab), etc., hot molecular markers EGFR (epidermal growth factor receptor), CycIinDl (cyclin Dl), COX-2 (cyclooxygenase 2), PCNA ( Proliferating cell nuclear antigen), VEGF (vascular endothelial growth factor) and so on all have certain clinical significance in judging the prognosis of esophageal cancer, but the above molecular markers mainly focus on the research on different tumor pathological stages, as well as on lymph node metastasis and tumor infiltration depth. There is a lack of research on molecular markers with different prognosis for the same pathological stage, the same operator, the same operation method, and the same postoperative treatment plan. There is a lack of research on early recurrence and metastasis of esophageal cancer after surgery. There is a lack of specificity. Molecular markers with high sensitivity and high sensitivity can be used to predict the occurrence, development and prognosis of esophageal cancer, and the process of esophageal cancer and development is the result of polygenic changes, and the molecular biological changes are not completely the same. The combination of molecular marker detection is helpful. To improve the sensitivity of judging the prognosis

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  • MiRNA marker associated with post-esophageal-cancer-operation early recurrence and prognosis and applications thereof
  • MiRNA marker associated with post-esophageal-cancer-operation early recurrence and prognosis and applications thereof
  • MiRNA marker associated with post-esophageal-cancer-operation early recurrence and prognosis and applications thereof

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Experimental program
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Effect test

Embodiment

[0028] 1. Sample selection

[0029] Two paraffin specimens were selected from the First Affiliated Hospital of Xinxiang Medical College from 2006 to 2007 with the same pathological stage and significantly different prognosis and survival. These two specimens were both paraffin specimens made from tumor samples of primary lesions surgically resected for the first time. At the same time, the normal esophageal mucosa 8 cm away from the edge of the tumor tissues of the two paraffin samples were selected as controls. In 2 cases, there was no distant metastasis diagnosed by physical examination and imaging before operation, and no radiation therapy or chemotherapy was received before operation. Both cases underwent esophageal cancer resection + thoracic and abdominal two-field lymph node dissection, and neck anastomosis was performed manually. Postoperative chemotherapy was performed twice, and postoperative pathological examination revealed well-differentiated squamous cell carcino...

experiment example 1

[0068] Experimental example 1. Verification of expression difference of has-miR-197 in patients with esophageal cancer with the same stage but different prognosis

[0069] 1. Sample selection

[0070] Selected 46 cases of paraffin wax specimens from the First Affiliated Hospital of Xinxiang Medical College from 2006 to 2009 with the same pathological stage and significant differences in prognosis and survival. There was no distant metastasis in the imaging diagnosis of the examination machine, and no radiation therapy or chemotherapy was received before operation. Among them, 18 cases were specimens of esophageal cancer that were alive 5 years after surgery and had a good prognosis, and 28 cases were specimens of esophageal cancer that had recurred early within 1 year after surgery and had a poor prognosis. The patient information is shown in Table 7. At the same time, the normal esophageal mucosa with the same pathological stage and 8 cm away from the edge of the tumor tissu...

experiment example 2

[0099] Experimental example 2. Analysis of has-miR-197 expression level and risk score method for early recurrence and prognosis of esophageal cancer

[0100] Kaplan-Meier was used to analyze the correlation between the expression level of has-miR-197 and the survival time, as shown in figure 2 shown. The analysis results showed that the expression level of has-miR-197 was significantly correlated with the survival time (p=0.030).

[0101] Cox single-factor risk analysis was performed on the prognosis, gender, age, TNM stage, postoperative T, N0-N1 stage, tumor size, degree of differentiation, and expression of has-miR-197 in patients with esophageal cancer. The results are shown in Table 9. Show. The results showed that the patient's prognosis (P=0.000), TNM stage (P=0.041), postoperative T (p=0.010), tumor size (p=0.006) and has-miR-197 expression (p=0.029) were significantly different from those of patients. The survival time was significantly correlated.

[0102] Tabl...

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Abstract

The invention discloses a miRNA marker associated with post-esophageal-cancer-operation early recurrence and prognosis and applications thereof. The miRNA marker is has-miR-197. The invention discloses the application of the miRNA marker in preparing auxiliary diagnostic kits for post-esophageal-cancer-operation early recurrence and prognosis. The invention also discloses the application of a primer of the miRNA marker associated with post-esophageal-cancer-operation early recurrence and prognosis in preparing auxiliary diagnostic kits for post-esophageal-cancer-operation early recurrence and prognosis. The invention also discloses an auxiliary diagnostic kit for post-esophageal-cancer-operation early recurrence and prognosis of a primer of the miRNA marker associated with post-esophageal-cancer-operation early recurrence and prognosis. According to the invention, a method is simple, the operation is convenient, and the has-miR-197 obtained through screening can accessorily judge the prognosis or death risks of patients with esophageal-cancers, thereby promoting the development of diagnosis and treatment of esophageal-cancers.

Description

technical field [0001] The invention relates to a miRNA marker related to the early recurrence and prognosis of esophageal cancer after operation, and also relates to the application of the marker, which belongs to the fields of genetic engineering and oncology. Background technique [0002] Esophageal cancer (Esophageal carcinoma, EC) is a common malignant tumor of the digestive tract, accounting for the second place in the death of tumors in various parts of the digestive system. The world dies from this disease every year, reaching 300,000 people, of which about 150,000 people die in China every year. It is the country with the highest incidence rate of esophageal cancer in the world. Although great progress has been made in chemotherapy, radiotherapy, and concurrent chemoradiotherapy for esophageal cancer in recent years, surgery is still the most important treatment for esophageal cancer. However, the treatment effect is not ideal, and the 5-year survival rate is only ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12Q1/68
Inventor 王天云赵宝生刘尚国杨晓煜赵春澎李汉臣
Owner 河南普诺易生物制品研究院有限公司
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