Application of trepang sulphated polysaccharide in preparation of drugs or products for resisting diabetes mellitus II
A diabetes drug, technology of sulfated polysaccharides, applied in the improvement or treatment of type II diabetes, sea cucumber sulfate polysaccharides in the field of prevention, can solve the problem of no sea cucumber sulfate polysaccharides, achieve significant treatment of type II diabetes, eliminate side effects, increase glucose The effect of tolerance
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Embodiment 1
[0017] The preparation method of embodiment 1 sea cucumber sulfate polysaccharide:
[0018] 1. Preparation of sea cucumber chondroitin sulfate and sea cucumber fucoidan sulfate: desilt and pulverize dried sea cucumber, degrease in 95% ethanol for 24 hours, and dry at room temperature. Add 3L 0.1M sodium acetate buffer solution (pH6.0), 10g papain, 2.92g EDTA solution and 1.21g cysteine solution to 100g dry matter, stir and react in a water bath at 60°C for 24h, then centrifuge the reaction mixture (4000g , 15min). Add 160mL of 10% CPC solution to the supernatant, leave it at room temperature for 24h, centrifuge (3000g, 15min), and discard the supernatant. Dissolve the precipitate in 1.5L 3mol / L NaCl: ethanol (100:15v / v) solution, then add 3L 95% ethanol solution, place at 4°C for 24h, centrifuge (3000g, 15min), use 3L 80% and 95% ethanol for precipitation Wash two to three times. Finally, the precipitate was dried at 60° C. for 2 hours, dissolved in distilled water, ultra...
Embodiment 2
[0022] Example 2: Preventive and therapeutic effects of sea cucumber chondroitin sulfate on type II diabetic mice
[0023] one. Materials and methods:
[0024] A mouse model of type II diabetes was established by high-sugar and high-fat feeding. C57BL / 6 male mice weighing 19–21 g were randomly divided into 6 groups: normal control group, model control group, positive control group, sea cucumber chondroitin sulfate (SC-CHS) low and high dose groups, high dose sea cucumber Chondroitin sulfate and rosiglitazone compound group, 10 rats in each group. The normal control group was fed with normal feed, the protein content in the formula was 20%, the fat content was 5%, and the starch content was 65%, and the mineral and vitamin complexes were all referred to the AIN-93 formula; the mice in other groups were fed with high-sugar For high-fat feed, the protein content in the formula is 20%, the fat content is 25%, the sucrose is 30%, and the mineral and vitamin complexes are all ref...
Embodiment 3
[0071] Example 3 Capsule preparation
[0072] Get 100g of sea cucumber sulfated polysaccharide prepared in Example 1, 20g of micropowdered silica gel, 380g of dextrin, and 500g of starch, mix well and pack into capsules, each capsule is 1g, which contains sea cucumber sulfated polysaccharide 100mg.
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