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Application of active ingredient namely loganin in dogwood in preparation of medicaments for treating diabetes

The technology of a diabetes drug and active ingredient is applied to the application field of loganin, the active ingredient in Cornus officinalis, in the preparation of diabetes drugs, which can solve the problems of large side effects, inability to alleviate chronic complications of diabetes, etc., and achieves improvement of three more and one less. Symptoms, good effect, obvious effect of hypoglycemic effect

Inactive Publication Date: 2013-05-22
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the chemical drugs used clinically to treat diabetes, such as sulfonylureas and biguanides, have a certain hypoglycemic effect, they cannot slow down the progression of chronic complications of diabetes due to relatively large side effects and no effect on the produced AGEs. Occurs, has certain limitations in the treatment of diabetes

Method used

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  • Application of active ingredient namely loganin in dogwood in preparation of medicaments for treating diabetes
  • Application of active ingredient namely loganin in dogwood in preparation of medicaments for treating diabetes
  • Application of active ingredient namely loganin in dogwood in preparation of medicaments for treating diabetes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The extraction method of active ingredient loganin monomer compound in the cornus officinalis of embodiment 1, its extraction method step is as follows:

[0020] (1) Extraction of Cornus officinalis by water extraction and alcohol precipitation:

[0021] Weigh about 5kg of Cornus officinalis coarse powder (passed through a 10-mesh sieve), add 6 times of water to soak overnight, then decoct 2 times, 1 hour each time, filter, combine water extracts, concentrate under reduced pressure to 1 / 5 of the original volume, Add 4 times the amount of absolute ethanol, stir well, refrigerate overnight, filter, recover the ethanol from the filtrate, concentrate under reduced pressure until it has no alcohol smell, and obtain the water-extracted alcohol-precipitated extract of Cornus officinalis;

[0022] (2) Enrichment of iridoid glycosides in Cornus officinalis extract by macroporous resin:

[0023] Take the Cornus officinalis extract obtained in step 1, load it into a D101 macropor...

Embodiment 2

[0028] The purity determination step of embodiment 2 loganin monomer compound

[0029] Determination by HPLC method. The conditions are chromatographic column: ThermoODSHypersil (4.6mm×150mm, 5μm); mobile phase: water-acetonitrile (88:12); flow rate: 1mL·min-1; injection volume: 20μL; column temperature: 35°C; detection wavelength: 240nm. Loganin (molecular formula: C 17 h 26 o 10 , molecular weight: 390.38) with a purity of 98.5%. The chemical structural formula of loganin is as follows: figure 1 It is the loganin HPLC chromatogram in the sample;

[0030] figure 2 The hydrogen spectrum of loganin in the sample

[0031]

Embodiment 3

[0032] Example 3 Effect of Loganin on AGEs Feed Compound STZ-induced Diabetic Mice

[0033] Prepare loganin monomer compound by the extraction method of embodiment 1 and carry out pharmacological experiment research, method is as follows:

[0034]Male 6-week-old C57BL / 6J mice, weighing 18-22g, were fed adaptively for 1 week, fasted for 12 hours before modeling, and injected intraperitoneally with Streptozotocin at a dose of 100 mg / kg on the first day and the fourth day, respectively. On the 8th day, the fasting blood glucose of the mice was measured, and the mice with a blood glucose value greater than 16.7mmol / L were selected as the successful mice for diabetes modeling. Randomly divided into model group, metformin group (0.2g / kg), low-dose loganin group (0.02g / kg), high-dose loganin group (0.1g / kg) according to blood glucose level, 10 rats in each group, and the above small Rats were fed with high AGEs diet. At the same time, another blank control group was set up. The dr...

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Abstract

The invention discloses a new medical application of loganin, and in particular relates to an application of the loganin in preparation of medicaments for treating diabetes, wherein the loganin is extracted from dogwood. The method for extracting the loganin comprises the following steps of: (1) performing water extraction and alcohol precipitation on dogwood coarse powder to obtain a dogwood water extraction and alcohol precipitation extracting solution; (2) loading the dogwood water extraction and alcohol precipitation extracting solution into a macroporous adsorption resin column, and performing enrichment to obtain dogwood iridoid glycosides; (3) performing chromatographic separation by using a silica gel column to obtain a loganin flow component; and (4) purifying to obtain a loganin monomeric compound. Pharmacodynamics experimental results prove that the loganin has the functions of reducing the content of blood sugar, serum AGEs and urokinase protein, raising the serum insulin level, relieving pathologic lesions of pancreas, kidney, liver, heart, testis and the like, and obviously improving a 'three more one less' symptom. Therefore, the loganin can be used for preparing the medicaments for treating the diabetes.

Description

technical field [0001] The invention relates to the new medical application of loganin, in particular to the application of loganin, the active ingredient in cornus officinalis, in the preparation of medicines for treating diabetes. Background technique [0002] Diabetes belongs to the category of "diabetes syndrome" in traditional Chinese medicine. Long-term diabetes can cause chronic complications of diabetes, mainly vascular disease. The nature of macrovascular disease is atherosclerosis, which mainly involves large blood vessels such as aorta and coronary artery, while the microvascular disease of diabetes mainly manifests as microvascular disease such as retina and kidney. Clinical statistics show that 40%-50% of diabetic patients develop retinopathy 10 years after onset; about 2% of patients are completely blind 15 years after onset; End-stage renal disease. Although the exact pathological mechanism of the occurrence and development of diabetic chronic complications...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P3/10
Inventor 许惠琴吕高虹杨欢赵腾斐狄留庆刘斌李伟吴佳蕾陶玉菡刘凯
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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