Rotifer killing medicine, and preparation method and application thereof

Abstract

The invention belongs to the biotechnical field, and concretely relates to a rotifer selective-killing medicine, and a preparation method and application thereof. The medicine is a polyol ester compound extracted from Celastraceae plants and having a structure represented by formula I shown in the specification. In the invention, the basic biological differences of animals and plants are compared, tissue organs and submicroscopic organelles which only exist in the animals and do not exist in microalgae are selected as main foci, the botanical medicine which can be degraded and has a small toxicity is screened, and the rotifer growth inhibition and spawning rate and hatching rate reduction are realized through selectively generating anorexia response, destroying the cell membranes and the organelle membranes of a digestion organ system and inhibiting the nerve-muscle excitatory junction potential. The rotifer killing medicine has the advantages of safe, rapidness and effective technology, and specificity of the rotifer killing and inhibition.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a rotifer-killing agent and its preparation method and application. Background technique [0002] Microalgae (including freshwater microalgae and seawater microalgae), as an important part of primary productivity in the biosphere, are the only bait for the entire growth process (or specific developmental stages) of aquatic animals in aquatic ecosystems or the bait for their bait organisms. At the same time, microalgae are close to 50% of plant species, and have diversity, complexity and particularity in terms of germplasm, ecological distribution, genetic information, biochemical composition, and metabolic pathways, which in turn determine their potential food, nutrition, health care and Pharmaceutical equivalents. It is possible to develop high value-added bioactive ingredients that are highly demanded by humans and have specific structures (such as β-carotene, astaxanthin, lutein ...

AI Technical Summary

Problems solved by technology

[0007] 1) Microalgae cultivation pretreatment technology methods, such as ozone, ultraviolet light, sodium hypochlorite, physical filtration, etc., mainly carry out pre-disinfection treatment on microalgae cultivation materials (water, fertilizer, gas) and cultivation facilities, which is currently a common disinfection at home and abroad 1. The purification method is mainly to reduce the introduction and diffusion of rotifers and their eggs. This technology is not selective, and it also causes damage to microalgae. It is only suitable for pre-cultivation treatment, and cannot be used to kill the in-cultivation process. Microalgae system infecting rotifers discovered

[0008] 2) Physical methods in the microalgae cultivation process, such as the use of microporous filtration and nylon mesh isolation to control the purity of algae species. This technology can effectively remove large algae-eating animals, but for small algae-eating rotifers and its eggs are difficult to eradicate, and the filtration speed is slow, so the application in large-scale production is limited

[0009] 3) Chemical methods in the cultivation process, rotifers and algae belong to eukaryotes, this feature makes it impossible for people to simply apply drugs (formaldehyde, ammonia, hydrogen peroxide and sodium hypochlorite, etc.) and technologies such as reducing pH, that is, these methods There is no specificity, and the treatment process not only controls and removes rotifers and their eggs, but also causes serious harm to microalgae

[0010] 4) Use heavy metals to control rotifers. The poisoning research on rotifers mainly focuses on Cu, Zn, Cd, Pb, etc. It is found that these metal ions are highly toxic to rotifers, and the 24h semi-lethal concentration is about 1mg / L , this method only considers the impact on rotifers alone, and does not consider the impact on the growth and reproduction of microalgae and product quality

[0011] 5) Organophosphorus, carbamic acid and other pesticides, the killing effect of these pesticides on rotifers depends on the effective concentration of poisons, and the impact on the growth and reproduction of microalgae and the quality of its products is also not considered

[0012] In addition, in the 1990s, a specific and selective virus and fungal technology method appeared, that is, Comps et al. isolated viruses and fungi (marine double RNA virus and streptochytrium) from dead rotifers in the farm, and tested Indoor infection with rotifers, evidence that these viruses and fungi can lead to reduced rotifer populations and reduced fecundity

It is expected that this method will be specific in the future, and may have good application prospects in the large-scale cultivation of microalgae, but it is still in the stage of laboratory research and development

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