Integrated detoxification method of vitis vinifera in test tube

Abstract

The invention relates to an integrated detoxification method of vitis vinifera in a test tube, belonging to the technique of plant propagation. The method comprises the following steps: (1) conducting water cultivating on dormant branches of the vitis vinifera to impel dormant buds to sprout, employing treated stem tips as explants for standby; (2) inoculating the cut vitis vinifera stem tips to a minimal medium added with 0.08-0.13mg.L of indolebutyric acid, 2.20-3.30mg.L of gibberellins, 5g.L of edible sugar and 8.0g.L of agar stripes for cultivating to remove a grapevine fanleaf virus and a grapevine leafroll associated virus; (3) controlling the cultivating temperature to be 45-48 DEG C, and cultivating for 51-57 days; and (4) when the stem tip grows to 1.0cm, cutting 0.03-0.05cm of stem tip to further carry on nontoxic seedling cultivation. Through the application of the integrated detoxification method, the removal rates of the grapevine fanleaf virus and the grapevine leafroll associated virus can reach 100.0%, and the survival rate of nontoxic seedlings can be more than 97.0%. The integrated detoxification method of vitis vinifera in the test tube can be directly applied to the industrialized production of vitis vinifera toxin-free seedlings.

Description

[0001] Technical field [0002] The present invention involves an integrated detoxification method in a grape test tube, which belongs to plant reproduction technology. Background technique [0003] In existing technology, grapes ( Vitis vinifera ) It is a deciduous vine -based plant for grapes. It is the second largest fruit in the world today. In the production of fruit products around the world, the production and cultivation area of grapes have always ranked first.Grapes have the functions of relieving fatigue, antiddy sterilization, improving allergic symptoms, anti -anemia, nourishing gastrointestinal, bile bile, diuretic swelling, tires, beauty and beauty, replenishment and excitement of brain nerves.Grapes not only have high value in terms of consumption and medicinal, but also have great economic and social benefits in the wine industry. [0004] Because the cultivation of grape seedlings is obtained through asexual reproduction, the virus in grapes will spread with non -s...

AI Technical Summary

Problems solved by technology

[0005] At present, grape detoxification mostly adopts methods such as single shoot tip tissue culture detoxification, single heat treatment, single micron grafting and meristem culture for detoxification. Although the detoxification effect is better, the stem tip, micron grafting and Detoxification of meristem culture requires tiny materials, and part of the work needs to be done under a microscope; while heat treatment can lead to abnormal growth of grape plants, decreased vigor and unsuitable for mass production due to the high temperature conditions of grape plants, humidity and light, etc. The detoxification method has the disadvantages of cumbersome steps, poor detoxification effect, and difficult operability.

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