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Fungus for promoting symbiotic germination of paphiopedilum hirsutissimum seed and application thereof

A technology of Paphiopediphyllum and seeds, which is applied in the field of fungi that promote the symbiotic germination of Paphiopediphyllum seeds, can solve the problems of lack of strains for symbiotic germination of Paphiopediphyllum seeds, and difficulty in separating and cultivating mycorrhizal fungi, achieving low cost and simple operation , the effect of promoting seedling development

Inactive Publication Date: 2013-05-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scholars at home and abroad have used the technology of symbiotic germination of orchid mycorrhizal fungi and their seeds to a variety of orchids. However, due to the difficulty in isolating and culturing mycorrhizal fungi of Paphiopedilum plants, there is still a lack of strains that can symbiotically germinate with Paphiopedilum seeds

Method used

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  • Fungus for promoting symbiotic germination of paphiopedilum hirsutissimum seed and application thereof
  • Fungus for promoting symbiotic germination of paphiopedilum hirsutissimum seed and application thereof
  • Fungus for promoting symbiotic germination of paphiopedilum hirsutissimum seed and application thereof

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preparation example Construction

[0020] The preparation method of oat medium: add 8g of oatmeal into distilled water, boil for 30 minutes, filter with gauze and collect the filtrate, make the volume to 1000ml with distilled water, then add 10g of agar and 0.05g of chloramphenicol, heat and stir until the agar dissolves, After subpackaging, sterilize with damp heat at 121°C for 20 minutes.

[0021] The preparation method of the mixed substrate: mix equal volumes of sand and vermiculite, and cover the surface with a layer of moss.

Embodiment 1

[0022] Embodiment 1, acquisition, identification and preservation of bacterial strains

[0023] 1. Obtaining the strain PhI17

[0024] Take the vegetative root segment of P.hirsutissimum collected in the field that grows well, and gently peel off the fluffy root of the root segment, and rinse it under tap water. Microscopic inspection under an optical microscope, select the root segments that form abundant mycelium clusters. On an ultra-clean workbench, soak in 75% alcohol for 30 seconds, then sterilize the root surface with 1% NaClO for 4 minutes, wash with sterile water for 3-4 times, and dry the water with sterile filter paper. Cut the root segment into thin slices with a thickness of about 0.5 mm with a sterilized razor blade, spread them on 1 / 5 PDA separation medium, and cultivate them in an incubator at 25 °C. After the hyphae grew out from the root slices, they were transferred to PDA medium for purification and culture, and finally stored at 4°C on the slope of the P...

Embodiment 2

[0034] Embodiment 2, bacterial strain PhI17 and the symbiotic germination of P. pachyderm seeds

[0035] 1. Acquisition of Pachyphyllum chinensis seeds

[0036] Collect the naturally strong, well-grown, full-bodied, seven or eight mature and uncracked capsules of Pachyphyllum pallidum in the field, and repeatedly wipe the surface of the capsules with 75% alcohol cotton to remove the fluff of the pericarp. Soak for 30s, then sterilize with 4.5% NaClO for 8min, wash with sterile water 3-4 times. Place the capsules in a petri dish with sterile filter paper placed in advance, hold the capsules with tweezers with one hand, and cut the fruit longitudinally with a scalpel with the other hand to take out the seeds. Take a small amount of seeds to observe the embryonic rate to ensure that the embryonic rate of the seeds used for the test is greater than 80%.

[0037] 2. Obtaining of agar block of bacterial strain PhI17

[0038] Pick the PhI17 colony and inoculate it on a PDA medium ...

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Abstract

The invention discloses a fungus for promoting the symbiotic germination of paphiopedilum hirsutissimum seeds and application thereof. The strain PhI17 provided by the invention is identified as Epulorhiza sp., the preservation number of the strain PhI17 is CGMCC No.6756. According to the invention, the strain PhI17 and the paphiopedilum hirsutissimum seed are subjected to co-culture, so that the germination of the paphiopedilum hirsutissimum seeds can be successfully promoted, the growth of seedlings can be further promoted, and leaves capable of photosynthesizing are grown; and the seedlings are transplanted to a matrix for culture, and the seedlings can all survive and well grow. The method provided by the invention can promote the germination of the paphiopedilum hirsutissimum seeds or is used for carrying out culture of paphiopedilum hirsutissimum seedlings, is easy to operate and low in cost. The fungus disclosed by the invention has important economic value on the artificial breeding of paphiopedilum hirsutissimum.

Description

technical field [0001] The invention relates to a fungus for promoting the symbiotic germination of Paphiopedilum pachyderm seeds and its application. Background technique [0002] Paphiopedilum is one of the most popular orchid plants with peculiar flower shape and gorgeous color. People often call Paphiopedilum "slipper orchid" and "cinderella". In the past ten years, due to rampant smuggling and habitat destruction, the number of wild Paphiopedilum has decreased sharply, and its distribution area has gradually shrunk. Many species have reached the verge of extinction. [0003] In order to alleviate the pressure on the collection of wild plants, protect endangered species, and meet people's needs for Paupelopera plants, scholars at home and abroad have conducted a lot of research on the artificial propagation of Paphiopedilum plants. At present, the aseptic sowing and tissue culture techniques of some Paphiopedilum species have been successful, but the growth of aseptic s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/00C12R1/645
Inventor 刘红霞孙晓颖毛益婷罗毅波
Owner BEIJING FORESTRY UNIVERSITY
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