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Bacillus subtilis YB-05, microbial preparation thereof, and application of Bacillus subtilis YB-05 or microbial preparation

A Bacillus subtilis, microbial preparation technology, applied in the directions of microorganisms, biocides, microorganism-based methods, etc., can solve problems such as lack of disease-resistant varieties of biocontrol preparations

Active Publication Date: 2013-05-01
河南新仰韶生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the control of this disease, chemical control is still the main method at present, and there is a lack of effective disease-resistant varieties and high-efficiency biocontrol agents. Using the antagonism between microorganisms to carry out biological control of wheat take-all has broad application prospects.

Method used

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  • Bacillus subtilis YB-05, microbial preparation thereof, and application of Bacillus subtilis YB-05 or microbial preparation
  • Bacillus subtilis YB-05, microbial preparation thereof, and application of Bacillus subtilis YB-05 or microbial preparation
  • Bacillus subtilis YB-05, microbial preparation thereof, and application of Bacillus subtilis YB-05 or microbial preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Isolation and screening of Bacillus subtilis YB-05

[0019] Soil sample collection: In the wheat rhizosphere of the wheat field in Tanzhuang Town, Shangshui County, five-point sampling was adopted. When sampling, the top 5 cm soil layer was shoveled off, 500 g of fresh soil samples were taken, and brought back with a fresh-keeping bag. After mixing the 5 soil samples, 500 g was taken as a soil sample (collection time: April 20, 2010; specific collection location: Tanzhuang Town, Shangshui County, Zhoukou City, Henan Province; collector: Yang Lirong).

[0020] Isolation and purification of Bacillus subtilis: Weigh 5g of soil sample, put it in a conical flask filled with 45mL of sterile distilled water, heat on low heat until the suspension boils, keep it for 15min, then stand still for 5min, draw the supernatant and dilute to 10 -2 and 10 -3 , the supernatants of different dilutions were spread on the LB plate medium. Place the culture medium upside down at 3...

Embodiment 2

[0023] Example 2 Identification of Bacillus subtilis YB-05

[0024] Morphological characteristics of strain YB-05: the strain YB-05 with better rescreening effect was cultured on LB culture plate at 28°C for 1 day, and the colony shape and color were observed. YB-05 grows well on LB medium, the colony is milky white, no pigment is seen, the colony shape is irregular, the surface has bulges, and forms irregular folds, (see figure 2 ). After cultivating for 24 days, a slight fishy smell was produced, and the bacteria colonized into a ball with mucus when provoked. Gram stain was positive (G + ).

[0025] Physiological and biochemical identification of strain YB-05: Refer to the methods of "Berger's Bacteria Identification Handbook (Eighth Edition" and "Common Bacteria Identification Handbook") to measure and observe some of its physiological and biochemical indicators.

[0026] It can be seen from Table 2 that strain YB-05 can liquefy gelatin, hydrolyze starch and reduce ni...

Embodiment 3

[0031] Embodiment 3 YB-05 microbial bacterial agent preparation method

[0032] (1) Strain cultivation and preparation Inoculate Bacillus subtilis YB-5 on the slant medium of TSA test tube, and culture at 28-35°C for 24-36 hours to obtain the bacteria;

[0033] (2) Seed solution preparation: Introduce the test tube bacteria prepared in step (1) into TSB culture medium, and shake and culture at 28-32°C for 12-16 hours to make seed solution;

[0034] (3) Fermentation medium preparation Take glucose, corn flour, soybean cake powder, and dipotassium hydrogen phosphate and add water to prepare a culture solution containing 1.2% glucose, 1.8% corn flour, 3% soybean cake powder, and 0.2% dipotassium hydrogen phosphate ;

[0035] (4) Liquid fermentation production After sterilizing the fermentation equipment and culture medium, inoculate the seed liquid into the culture liquid prepared in step (3) at an inoculation amount of 5%, at 27-34°C, at a speed of 180rpm, and at a pH of 8.0 A...

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Abstract

The invention relates to a Bacillus subtilis strain YB-05, and a preparation method of an inoculant of the Bacillus subtilis strain YB-05. The Bacillus subtilis strain YB-05 is preserved at China General Microbiological Culture Collection Center, and has a preservation number of CGMCCNO.6498. The microbial inoculant is a pulvis prepared through the spray dry preparation of a fermentation solution of the Bacillus subtilis strain YB-05. The YB-05 strain and its inoculant have the advantages of wide antibacterial spectrum, good antimicrobial effect, and strong inhibition effects on the wheat take-all disease, tomato Botrytis cinerea, wheat sheath blight fungi, Phytophthora melonis and the like; and the inoculant has the advantages of high efficiency, non-toxicity, simple use, and no environmental pollution.

Description

technical field [0001] The invention relates to a biocontrol microorganism, in particular to a bacillus subtilis YB-05, its microbial preparation and its application. Background technique [0002] Bacillus subtilis ( Bacillus subtilis ), has fast growth and reproduction, simple nutrition, can produce heat-resistant and stress-resistant spores, is harmless to humans and animals, has good environmental compatibility, can inhibit various plant diseases, and is not easy to make crops resistant to pesticides. Moreover, the batch production process is simple, the cost is also low, the application is convenient, the storage period is long and the like, therefore, it is an ideal biocontrol microorganism. At present, the fungus has shown good disease control effect on cucumber, pepper, rice, wheat, corn and other crops. In terms of biological control of plant fungal diseases, Bacillus subtilis A-13 developed in Australia has a good control effect on wheat carrot blight and other so...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P1/00C12R1/125
Inventor 杨丽荣薛保国全鑫马毅辉
Owner 河南新仰韶生物科技有限公司
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