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Bacillus subtilis capable of producing high-yield protease and application of bacillus subtilis

A Bacillus subtilis, protein technology, applied in application, bacteria, microorganism-based methods, etc., can solve the problems of weak practical application effect, easy to contaminate bacteria, industrialization development limitation, etc. Effect

Inactive Publication Date: 2013-04-24
QINGDAO HAIZHONGDA BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on Bacillus microecological preparations in my country is relatively late, and it has only entered into industrialized research and development in recent years. Moreover, the strains of these products are relatively single, poor in functionality, and easy to infect bacteria. The actual use of microecological products in animal husbandry The application effect is very little, and the industrialization development is restricted

Method used

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  • Bacillus subtilis capable of producing high-yield protease and application of bacillus subtilis
  • Bacillus subtilis capable of producing high-yield protease and application of bacillus subtilis
  • Bacillus subtilis capable of producing high-yield protease and application of bacillus subtilis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Obtaining of Bacillus subtilis (Bacillus subtilis) Hzd-bs-001 and detection of its culture conditions

[0015] 1. Screening and identification of Bacillus subtilis Hzd-bs-001

[0016] 1) Screening of Bacillus subtilis Hzd-bs-001

[0017] (1) Weigh 1g of soil from the soil where brewer’s grains have been piled up all the year round in Tsingtao Brewery, put it in 100ml of sterile water, stir it evenly with a glass rod, then shake it on an oscillator for 30min to disperse the microbial cells, and let it stand for 20s~30s , its aqueous solution is 10 -2 Diluent, then draw 1ml of diluent into a test tube of 9ml sterile water, shake and mix well, which is 10 -3 Diluent, then draw 1ml 10 -3 The diluent was shaken and mixed in 9ml sterile water, which was 10 -4 dilution, to make 10 -2 、10 -3 、10 -4 serial dilutions.

[0018] (2) Number the culture medium plates (set 3 replicates for each number), and use a sterilized inoculation loop to dip one loop of each series of di...

Embodiment 2

[0032] Salt tolerance test of strains

[0033] Under sterile conditions, Bacillus subtilis Hzd-bs-001 was cultured with 5% inoculum in different salinity fermentation media at 230r / min, shaking at 35°C, and its OD600 value was measured every 2h, as shown in Table 2. Show. The test results show that Bacillus subtilis Hzd-bs-001 has strong salt tolerance and is suitable for feed production requirements.

[0034] Table 2 Salt tolerance test of Bacillus subtilis (Bacillus subtilis) Hzd-bs-001

[0035]

Embodiment 3

[0037] Temperature tolerance test of bacterial strains

[0038] Under sterile conditions, Bacillus subtilis Hzd-bs-001 was placed in the same fermentation medium at 5% inoculum size, 230r / min, shaking culture at different temperatures, and its OD600 value was measured every 2h, as shown in Table 2 . The test results showed that Bacillus subtilis Hzd-bs-001 grew most vigorously at 35°C, and also had better growth at 45°C, which was suitable for feed production requirements.

[0039] Table 3 Temperature tolerance test of Bacillus subtilis (Bacillus subtilis) Hzd-bs-001

[0040]

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Abstract

The invention relates to bacillus subtilis Hzd-bs-001 with the collection number of CGMCC No.6928. The bacillus subtilis Hzd-bs-001 can be applied to biological fermentation feed, microecological preparations and animal health-care products and is probiotics beneficial for growth of animals. The test indicates that the bacillus subtilis Hzd-bs-001 has high capacity of decomposing protein, can obviously increase the utilization ratio of animal protein, can effectively reduce the morbility of animal diseases, can balance intestinal bacterial colonies, particularly has extremely obvious effect of preventing and treating piglet diarrhea, improves the immunity of the animals, and promotes healthy and quick growth of the animals. The invention has the advantages that the bacillus subtilis Hzd-bs-001 is separated from soil for stacking beer vinasse for many years and has high degradation rate on biomass with high protein content such as beer vinasse and soy sauce dregs, so high-activity protease can be generated in the fermentation process; and the bacillus subtilis Hzd-bs-001 can effectively prevent and treat the piglet diarrhea.

Description

technical field [0001] The invention relates to bacterial strains, in particular to a high protease-producing Bacillus subtilis and its application in feed fermentation Background technique [0002] Bacillus is widely distributed in nature, and is generally an aerobic or facultative anaerobic Gram-positive bacillus, which can produce round, oval or columnar endophytic spores in the cell. There is a cortical layer in the spore wall, which is highly resistant to heat, ultraviolet rays, ion radiation and antibiotics, and can withstand feed processing, storage, acid resistance, high temperature, extrusion resistance, etc. Research in Feed. [0003] Bacillus subtilis has a strong ability to produce protease, cellulase, and amylase, and can secrete a variety of antibiotics and other secondary metabolites; it can regulate the balance of intestinal flora in animals through intestinal space occupation, and improve the intestinal micro-ecological environment , promote animal health;...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/14A23K1/06A23K1/16A23K1/18C12R1/125A23K10/38
CPCY02P60/87
Inventor 栾锦涛陈继伟黄玲玲张盼盼
Owner QINGDAO HAIZHONGDA BIOTECH DEV
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