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Entomopathogenic nematodes insect corpse agent

A technology for entomopathogenic nematodes and insect carcasses, applied in the direction of insecticides, biocides, animal repellents, etc., can solve the problems of entomopathogenic nematodes not being resistant to drying, limiting the field application of entomopathogenic nematodes, and unstable field control effects , to achieve the effect of increasing the killing rate, reducing cost input, and avoiding insect cocooning

Inactive Publication Date: 2013-04-24
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At the same time, entomopathogenic nematodes are usually sprayed onto the soil surface, and entomopathogenic nematodes are not resistant to drying and ultraviolet radiation, which further affects the activity and virulence of entomopathogenic nematodes, and the effect and duration of entomopathogenic nematodes in the field are related to the survival time of the injected nematodes , due to the above-mentioned limitations in the direct application of entomopathogenic nematode solutions in the field for underground pest control, the field control effect is unstable, which greatly limits the field application of entomopathogenic nematodes

Method used

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  • Entomopathogenic nematodes insect corpse agent

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1. Entomopathogenic nematode cadaveric agent is used for preventing and treating field underground pest-leek leek maggot (Shandong Shouguang)

[0021] Experiments were arranged in a greenhouse leek field in Shouguang, Shandong. Select 5 leek plots (150m2 / plot) to apply insecticide, with an average of 1 insecticide per square meter. The specific application method is to dig a 3cm-5cm hole between the two rows of leeks, put the insect corpse agent into the hole and bury it with soil. Water once within 7 days after applying the insecticide, and the nematodes in the insecticide can be released into the soil. In addition, 5 adjacent leek plots (150m2 / plot) were selected from each farmer, and the chemical pesticide phoxim was used to control leek maggots according to the farmers' habits, and the dosage was 4.5kg / mu, as a control.

[0022] It can be clearly observed that the leeks in the treatment group are growing well, and there are not many green and yellow lea...

Embodiment 2

[0025] Embodiment 2. Entomopathogenic nematode cadaver agent is used for preventing and treating underground pests in the field-leek leek maggot (Tianjin Baodi)

[0026] Experiments were set up in open-field leek fields in Baodi, Tianjin. Select 4 leek plots (15m2 / plot) to apply insecticide, with an average of 1 insecticide per square meter. The specific application method is to dig a 3cm-5cm hole between the two rows of leeks, put the insect corpse agent into the hole and bury it with soil. Water once within 7 days after applying the insecticide, and the nematodes in the insecticide can be released into the soil. In addition, 5 adjacent leek plots (15m2 / plot) were selected, and the chemical pesticide phoxim was used to control leek maggots according to farmers' habits, and the dosage was 4.5kg / mu, as a control (CK).

[0027] It can be clearly observed that the leeks in the treatment group are growing well, while the leaves of the leeks in the chemical pesticide group are wi...

Embodiment 3

[0030] Embodiment 3. Entomopathogenic nematode cadaver agent is directly used in the prevention and treatment of underground pests in the field - grubs (Taizhou, Jiangsu)

[0031] Collect grubs and soil (the general term for larvae of the family Coleoptera Scarabidae) in the vegetable plots for planting shallots and taro in Hailing Agricultural Park, Taizhou City, Jiangsu Province, and bring them back indoors. Put the soil in two cut-off mineral water bottles, fill with 100 grams of soil, and 3-4 grubs. In the experiment, the entomopathogenic nematode nematodes treatment and the control were set, and the entomopathogenic nematode treatment was put in at a level of 1 nematode per bottle. After 4 days, the investigation results showed that after the entomopathogenic nematodes were treated with the dead agent, grubs could be effectively controlled (Table 4).

[0032] Table 4 The killing effect of entomopathogenic nematode cadavers on grubs (Taizhou, Jiangsu)

[0033]

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Abstract

The invention aims to establish a preparation method of an entomopathogenic nematode insect corpse agent, and provides a novel application method of entomopathogenic nematodes in the field. In view of the limitations of low activity and intolerance of nematode to drying UV irradiation in direct application of entomopathogenic nematodes in the field, the invention uses insects (Galleria mellonella etc.) as a carrier; the host insect Galleria mellonella is infected by an entomopathogenic nematodes aqueous suspension in a laboratory; 36h later, the Galleria mellonella are all died and prepared into an insect corpse agent; and the insect corpse agent is then propagated at room temperature for at least 4 days, and directly applied in the field to control plant underground pests (Chinese chive maggot, Bradysia odoriphaga), so as to replace a traditional chemical pesticide to control underground pests and reduce pesticide investment. The invention effectively solves the defects of direct application of entomopathogenic nematodes, and has advantages of easy manufacture, convenient application, obvious effect and benefit to popularization.

Description

technical field [0001] The invention relates to a method for effectively controlling soil harmful insects by using an entomopathogenic nematode corpse agent formed from an entomopathogenic nematode-insect host. Background technique [0002] According to reports by the World Health Organization and the United Nations Environment Program, more than 1 million people are poisoned by pesticides every year in the world, of which 20,000 people die. There are 67,000 pesticide poisoning accidents in the United States every year, and the situation is even more serious in developing countries. [0003] Pesticide pollution of vegetables in our country has become a livelihood issue that residents are strongly concerned about. In particular, the detection rate and exceeding standard rate of pesticides on leeks in our country are above 50%. There are frequent pesticide poisoning accidents, and food safety is worrying. As we all know, the long-term and high-frequency use of chemical pestic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P7/04
Inventor 阮维斌宋东民王欣赵东辰
Owner NANKAI UNIV
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