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Complementary DNA (cDNA) sequence of tobacco NtFT1 genes and transient expression thereof for inducing tobacco early blossoming

A technology of transient expression and genome sequence, applied in DNA preparation, recombinant DNA technology, genetic engineering, etc., can solve problems such as limited applications

Active Publication Date: 2013-04-17
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Description
  • Claims
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Problems solved by technology

However, because the FT gene is protected by international patents, the application of this gene by tobacco breeders in my country is limited.

Method used

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  • Complementary DNA (cDNA) sequence of tobacco NtFT1 genes and transient expression thereof for inducing tobacco early blossoming
  • Complementary DNA (cDNA) sequence of tobacco NtFT1 genes and transient expression thereof for inducing tobacco early blossoming
  • Complementary DNA (cDNA) sequence of tobacco NtFT1 genes and transient expression thereof for inducing tobacco early blossoming

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Embodiment Construction

[0025] The following examples facilitate a better understanding of the present invention, but do not limit the present invention.

[0026] Any improvement or substitution on the basic spirit of the present invention still belongs to the scope of protection required by the claims of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified.

[0027] The test reagents used in the following examples are conventional biochemical reagents unless otherwise specified.

[0028] Acquisition of cDNA sequence information of NtFT1 gene

[0029] Firstly, using the amino acid sequence of the Arabidopsis FT gene (AEE34381.1) as the search sequence, the Genbank tobacco GSS database was compared with the tblastn program, and the tobacco genome sequence with the highest consistency (FH969747.1) was obtained, with a consistency of 68%. . The sequence was used to search the sequencing database of the China Tobacco Genome Proje...

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Abstract

The invention relates to a complementary DNA (cDNA) sequence of tobacco NtFT1 genes and transient expression thereof for inducing tobacco early blossoming, in particular to NtFT1 genes of tobacco FT (Flowering locus T) and application of the NtFT1 genes in inducing the tobacco early blossoming, and belongs to the field of genes in molecular biology. The invention discloses a full-length coded sequence of the NtFT1 genes derived from tobacco for controlling the plant blossom time and a protein sequence coded by using the full-length coded sequence. The NtFT1 genes are obtained by performing Blastn comparison and splice site analysis on the data searched from China tobacco genome sequencing planning data through homologous sequence searching. According to the cDNA sequence, a method for performing semi-quantitative polymerase chain reaction (PCR) or reverse transcription-polymerase chain reaction (RT-PCR) on NtFT1 gene specific primers is designed to clone the genes. According to the invention, a potato virus X (PVX) virus expression vector of the NtFT1 genes is also constructed, and the constructed virus expression vector is used for performing diafiltration and infestation on flue-cured tobacco varieties including Hongda and Yunnan tobaccos 87 and K326 through agrobacterium, and can induce the early blossoming of the varieties to prove that the genes have a function of inducing the early blossoming of cured tobaccos. When a PVX virus transient expression system of the NtFT1 genes is used for tobacco breeding, the growing time of the tobaccos can be shortened, and the fixed number of years for breeding a new tobacco variety is reduced.

Description

technical field [0001] The present invention is a cDNA sequence of tobacco NtFT1 gene and its transient expression to induce tobacco early flowering, specifically relates to tobacco FT (Flowering locus T) gene NtFT1 and its application in inducing tobacco early flowering, and belongs to the field of genes in molecular biology . Background technique [0002] Plant flowering is a very complex process, regulated by external environmental factors and internal gene expression. Studies on the molecular mechanism of Arabidopsis flowering have shown that the flowering of Arabidopsis is controlled by four pathways, namely, photoperiod, autonomous pathway, vernalization pathway and gibberellin pathway, and the signal of the photoperiod pathway is transmitted to FT ( Flowering Locus T), vernalization pathway and autonomous pathway signals are transmitted to FT through FLC (Flowering Locus C), and the expression of FT gene induces the expression of floral meristem-specific gene AP1, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/10C12N15/84A01H5/00
Inventor 高玉龙谢贺肖炳光李永平
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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