Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Improved flagellin serving as immunologic adjuvant, and preparation method and application of improved flagellin

A flagellin and flagellum technology, applied in the biological field, can solve the problems of no change in adjuvant ability, adverse reactions, restricting application prospects, etc., and achieve the effect of significant adjuvant activity and weakened immune response.

Active Publication Date: 2014-07-09
YANGZHOU UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, a recent study found that the mixture of flagellin and ovalbumin (OVA) immune TLR5 (- / -) The adjuvant ability to induce the humoral immune response of OVA protein did not change after the treatment of mice, thus inferring that the natural response mediated by TLR5 is not a necessary condition for the adjuvant effect of flagellin
[0006] In addition, after flagellin acts on the TLR5 receptor, the strong natural immune response stimulated can lead to systemic adverse reactions, such as sepsis, etc.
The side effects of flagellin on the body restrict its application as an adjuvant

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Improved flagellin serving as immunologic adjuvant, and preparation method and application of improved flagellin
  • Improved flagellin serving as immunologic adjuvant, and preparation method and application of improved flagellin
  • Improved flagellin serving as immunologic adjuvant, and preparation method and application of improved flagellin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1. Cloning of fliC gene of Salmonella typhimurium and construction of recombinant plasmid

[0064] A site-directed mutation of amino acid I→A at position 411 of flagellin fliC gene was inserted into the prokaryotic expression vector pTrc99a to construct a recombinant plasmid pTrc99a-fliC-411.

[0065] 1. Primers design and gene amplification

[0066] Design primers according to the DNA sequence of Salmonella typhimurium ATCC14028s in GenBank, and the upstream primer is 5'-ATA CCATGG CACAAGTCATTAAT-3' (SEQ ID NO: 1), the downstream primer is 5'-TCA AAGCTT AACGCAGTAAAGAGAG-3' (SEQ ID NO: 2), the upstream and downstream primers have Nco Ⅰ and HindIII sites respectively, the upstream primer contains the initiation codon ATG, and the downstream primer contains the fliC gene stop codon. The genome was extracted from Salmonella typhimurium ATCC14028s as a template, and the sequences of SEQ ID NO: 1 and SEQ ID NO: 2 were used as amplification primers. The fliC gene fragment was ampl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides improved flagellin serving as an immunologic adjuvant, and a preparation method and an application of the improved flagellin. A salmonella typhimurium flagellin mutant of the improved flagellin has bioactivity of flagellin, and contains I411A mutation compared with the wild salmonella typhimurium flagellin. The salmonella typhimurium flagellin mutant of the improved flagellin can weaken TLR5 (toll-like receptor) mediated natural immune response, and has significant adjuvanticity.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to an improved flagellin that can be used as an immune adjuvant and its preparation and application. Background technique [0002] Flagellin contains 494 amino acids and has 4 spherical domains (D0, D1, D2 and D3), among which D0 and D1 domains are highly conserved. [0003] The amino acid sequences of flagellin from various bacteria are available in the NCBI GenBank database. Among them, known from Salmonella typhimurium I (S. Typhimurium 1), Helicobacter pylori (H. Pylori), Vibrio cholerae (V. Cholera), S. marcesens (S. marcesens), Freund's dysentery S. flexneri, T. Pallidum, L. pneumophila, B. burgdorferei, Clostridium difficile (C. difficile), Rhizobium alfalfa (L. pneumophila) R.meliloti), A.tumefaciens, R.lupini, Bartonella carinii (B.clarridgeiae), Proteus mirabilis (P.Mirabilis), Bacillus subtilus (B.subtilus) ), Listeria monocytogenes (L.monocytogenes), Pseudomonas aeruginosa (P.ae...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/255C12N15/31C12N15/63C12N1/21A61K39/39C12R1/42
CPCY02A50/30
Inventor 焦新安潘志明张磊孙林杨芸康喜龙王静
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com