Method for preparing salvianolic acid A

A technology of salvianolic acid and salvia miltiorrhiza, applied in the field of salvianolic acid A preparation, can solve the problems of many conversion by-products, low yield of salvianolic acid A, uncontrollable conversion process, etc., and achieve the effect of improving the conversion rate

Active Publication Date: 2013-04-17
JIANGZI QINGFENG PHARMACEUTICALS INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] But the conversion process that above-mentioned prior art adopts can't be controlled, and conversion by-product is many, and the productive rate of

Method used

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  • Method for preparing salvianolic acid A
  • Method for preparing salvianolic acid A
  • Method for preparing salvianolic acid A

Examples

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Embodiment 1

[0059] Take Danshen medicinal material, crush it into 6-mesh granules, add 7 times the amount of 92°C water each time, warm soak and extract 3 times, and stir at a speed of 25 rpm, and warm soak and extract for 3 hours each time; the extract is concentrated under reduced pressure to relative Density 1.20 (60°C), add ethanol to make the alcohol content at 70%, let it stand, filter, the filtrate is decompressed to recover ethanol and concentrate until it has no alcohol smell; add water to dilute to contain salvianolic acid B20mg per 1ml, use 10% for aqueous solution Adjust the pH to 4.0 with sodium hydroxide, add 0.5% ZnCl 2 As a catalyst, heat and transform at 120°C for 4 hours, adjust the pH value of the transformation liquid to 2.5 with 20% phosphoric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 3 mg of salvianolic acid A per 1 ml, and perform HPD-100 macroporous resin column chromatography For separation, the ratio of the loading amount of ...

Embodiment 2

[0061] Take Salvia miltiorrhiza, cut into decoction pieces, add 8 times the amount each time, soak in water at 90°C for 3 times, stir at a speed of 20 rpm, and extract with warm soaking for 2.5 hours each time; the extract is concentrated under reduced pressure to a relative density of 1.18 (60 ℃), add ethanol to make the alcohol content at 75%, let it stand, filter, and the filtrate reclaims ethanol under reduced pressure and concentrates to no alcohol smell; add water to dilute to contain salvianolic acid B15mg per 1ml, adjust the aqueous solution with 10% potassium hydroxide pH to 4.0, add 0.6% FeCl 3 As a catalyst, heat conversion at 120°C for 3.5 hours, adjust the pH value of the conversion liquid to 2.5 with 15% hydrochloric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 5 mg of salvianolic acid A per 1 ml, pass through HPD-100 macroporous resin column layer Analysis and separation, the ratio of the loading amount of salvianolic acid A to...

Embodiment 3

[0063] Take the Danshen medicinal material, crush it into particles with a diameter of about 2mm, add 9 times the amount each time, soak in water at 85°C for 2 times, and stir at a speed of 30 rpm, and extract with warm soaking for 3.5 hours each time; the extract is concentrated under reduced pressure to a relative density 1.14 (60°C), add ethanol to make the alcohol content at 75%, let it stand, filter, and recover the ethanol from the filtrate under reduced pressure and concentrate until there is no alcohol smell; add water to dilute to 18mg per 1ml of salvianolic acid B, and use 10% carbonic acid in the aqueous solution Adjust pH to 5.2 with sodium, add 0.6% AlCl 3 As a catalyst, heat conversion at 123°C for 4.5 hours, measure the pH value of the conversion liquid to 2.8 with 15% nitric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 6 mg of salvianolic acid A per 1 ml, pass through HPD-100B macroporous resin column layer Analysis and separa...

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Abstract

The invention relates to a method for preparing salvianolic acid A. According to the method, salvia miltiorrhiza extract is prepared from salvia miltiorrhiza medicinal materials and is subjected to vacuum drying, spray drying or microwave vacuum drying after the salvia miltiorrhiza extract is subjected to catalytic conversion and the steps of separation, elution and the like, thereby obtaining the salvianolic acid A. The method for preparing the salvianolic acid A, provided by the invention, is very applicable to industrial application, lower in production cost and novel.

Description

technical field [0001] The invention relates to a preparation method of salvianolic acid A. technical background [0002] Salvia miltiorrhiza preparation is the basic treatment drug for cardiovascular and cerebrovascular diseases in my country. Because of its definite curative effect, Danshen has become one of the traditional Chinese medicines with the largest consumption, the highest sales, the most preparation factories and the most complete clinical dosage forms in China. There are two main types of effective chemical components in Danshen: fat-soluble tanshinone compounds and water-soluble phenolic compounds. Studies have shown that salvianolic acids have significant activities in anti-liver damage, anti-atherosclerosis and apoptosis, and improvement of memory dysfunction. Among them, Salvianolic acid A (Salvianolic acid A) has the strongest antioxidant activity. [0003] The structure of salvianolic acid A is as follows: [0004] [0005] Although in the prior art...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/32
Inventor 杨小玲吕武清刘地发程帆廖祝元曾发林
Owner JIANGZI QINGFENG PHARMACEUTICALS INC
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