Method for extracting lipide from hippocampus or syngnathus bone meal with supercritical carbon dioxide

Abstract

The invention discloses a method for extracting lipide from hippocampus or syngnathus bone meal with supercritical carbon dioxide. The method is characterized by comprising the following steps: (1) freezing hippocampus japonicus or syngnathus raw material at the temperature of 40 DEG C below zero, crushing the raw material, filtering through a 60-mesh screen, and taking the filterate to obtain the hippocampus or syngnathus bone meal; and (2) loading the hippocampus or syngnathus bone meal into a closed extraction kettle for being extracted by supercritical carbon dioxide fluid at the temperature of 45-60 DEG C and the pressure intensity of 25-40 MPa, adjusting the flow rate of carbon dioxide to 10-25L / h after 10 min of static extraction, then enabling the extract to enter a separation kettle of 60-80 DEG C together with carbon dioxide after 50-140 min of dynamic extraction, and collecting the extract to obtain the product. The method provided by the invention has the advantages that the extraction rate is high, the selectivity is good, no organic solvent remains, extraction and separation are integrated and the lipide is prevented from oxidative deterioration in the extracting process.

Description

technical field [0001] The invention relates to a method for extracting bone meal lipids, in particular to a method for extracting hippocampus or sea dragon bone meal lipids with supercritical carbon dioxide. Background technique [0002] As a medicine, hippocampus has a long history in our country. As early as Liang Dynasty Tao Hongjing’s "Compendium of Materia Medica", it has been recorded that "it is warm in nature and sweet in taste, returns to the liver and kidney meridian, has the functions of warming kidney and strengthening yang, reducing swelling and stagnation, and promoting birth for women. It can treat diseases such as respiratory system disorders, sexual dysfunction, insomnia, fatigue, sore throat, skin diseases and dystocia" when it is called "water horse". In 739 AD, Chen Zangqi used the name of hippocampus for the first time in "Compendium of Materia Medica". It is recorded in "Compendium of Materia Medica" that "it is mainly used for dystocia and blood gas p...

AI Technical Summary

Problems solved by technology

[0005] The existing hippocampus or sea dragon bone meal lipids are extracted based on traditional solvent extraction methods, most of which use Soxhlet extraction or rapid lipid extraction methods. The above lipid extraction methods all require heating of the solvent, and the operation process is complicated. Moreover, the lipids extracted by the solvent extraction method are not only poor in selectivity, and the lipids are easily oxidized during the heating process, but also cause a certain degree of heavy metal residues or exceed the standard, and the residual solvents will pollute the environment. Hippocampal lipids for experimental analysis, not suitable for production applications

CO 2 Supercritical fluid extraction technology is the use of supercritical CO above the critical temperature and critical pressure, between the gaseous state and the liquid state. 2 Fluid as an extraction solvent is an extraction technology widely used in medicine, food, spices, petrochemical and other fields. At present, there are no relevant research reports on the use of supercritical carbon dioxide fluid to extract lipids from hippocampus or sea dragon bone meal at home and abroad.

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