Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing high-purity astaxanthin extract

An astaxanthin and extract technology, which is applied in the field of preparing high-purity astaxanthin extract, can solve problems such as inability to realize industrialized production, and achieve the effects of good promotion and application prospect, good health care function, and high development value.

Inactive Publication Date: 2013-04-03
NINGBO HONGLONG BIO TECH
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although large-scale preparative chromatography can achieve the separation of astaxanthin monomer, it cannot achieve industrial production in the short term

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing high-purity astaxanthin extract
  • Method for preparing high-purity astaxanthin extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, a kind of method for preparing high-purity astaxanthin extract, carry out following steps successively:

[0037] 1) Take 1mL of natural astaxanthin oil produced by Ningbo Honglong Biotechnology Co., Ltd. and dilute it with 5mL of absolute ethanol to obtain the diluted astaxanthin crude extract;

[0038] Take 1 mL of the diluted astaxanthin crude extract and add it to 4 mL of 12 mmol / L NaOH-absolute ethanol solution, mix well and place it in a dark place for saponification at room temperature (25°C) for 1 hour to obtain a saponified solution;

[0039] 2) Add the saponified solution obtained in step 1) into the dichloromethane-water solution (obtained by mixing 5mL of dichloromethane+5mL of water), mix thoroughly, and then let stand to separate and discard the upper liquid. The lower liquid was concentrated at 45°C under reduced pressure (0.09MPa) to obtain an extract (about 200mg);

[0040] 3) Dissolve the extract obtained in step 2) in 1 mL of n-hexane:ac...

Embodiment 2

[0062] Embodiment 2, a kind of method for preparing high-purity astaxanthin extract, carry out following steps successively:

[0063] 1) Take 0.1mL of natural astaxanthin oil produced by Ningbo Honglong Biotechnology Co., Ltd. and dilute it with 0.5mL of absolute ethanol to obtain a crude astaxanthin extract after dilution;

[0064] Take 0.1 mL of the diluted astaxanthin crude extract and add it to 0.4 mL of 6 mmol / L NaOH-absolute ethanol solution, mix well and place it in a dark place for saponification at room temperature (30°C) for 0.5 h to obtain a saponified solution;

[0065] 2) Add the saponified solution obtained in step 1) into a solution of dichloromethane-water (2mL+1mL), mix thoroughly, and then let stand to separate layers, and discard the upper layer liquid. The lower liquid was concentrated under reduced pressure at 40°C to obtain an extract;

[0066] 3) Dissolve the extract obtained in step 2) in 1 mL of n-hexane:acetone=4:1 (volume ratio) solution, and load a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing a high-purity astaxanthin extract. The method comprises the following steps in sequence: (1) taking a crude extract of astaxanthin, diluting with absolute ethyl alcohol, adding a NaOH- absolute ethyl alcohol solution with concentration of 6-12 mmol / L, and then placing at a dark place for saponification at room temperature; (2) adding the obtained saponification solution into a methylene dichloride-water solution, standing for layering, and concentrating the lower layer liquid to obtain an extractum; (3) dissolving the obtained extractum into a solution with the volume ratio of normal hexane to acetone being 4 to 1, and applying onto a silicagel column; sequentially performing the following elution: taking the solution with the volume ratio of normal hexane to acetone being 4 to 1 as the elution solution I for elution to obtain a yellow fraction, taking the solution with the volume ratio of normal hexane to acetone being 1 to 1 as the elution solution II for elution to obtain an orange fraction, and eluting with methyl alcohol till the silicagel column is colorless; and (4) performing vacuum concentration on the orange fraction, thereby obtaining an aubergine extractum which is the high-purity astaxanthin extract.

Description

technical field [0001] The invention belongs to the separation and purification of natural products, and mainly relates to a method for preparing high-purity astaxanthin extract. Background technique [0002] Astaxanthin is a red carotenoid with the chemical name 3,3’-dihydroxy-β,β’-carotene-4,4’-dione and the molecular formula C 40 h 52 o 4 (Formula 1). Astaxanthin has a wide range of uses in industry: first, astaxanthin has health functions such as anti-oxidation, lowering blood fat, anti-tumor, and anti-aging, and can be used in food, health care products, pharmaceuticals and cosmetics industries; second, astaxanthin Astaxanthin is a good food coloring agent, which can be used as a food coloring agent; thirdly, astaxanthin can also be used as a feed additive in the cultivation of ornamental fish and shrimp to make its color more vivid. In terms of sources, astaxanthin can be either artificially synthesized or extracted from living organisms. The application of artifi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07C403/24C09B61/00
Inventor 黄胜奎马建陈黎明
Owner NINGBO HONGLONG BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com