Bicistronic mRNA coexpression gene transporter and preparation method thereof
A co-expression gene and gene transfer technology, applied in the biological field, can solve rare problems such as construction and application
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0059] The embodiments of the present invention will be described in further detail below in conjunction with the accompanying drawings: It should be emphasized that the embodiments of the present invention are illustrative rather than restrictive, and should not limit the protection scope of the present invention.
[0060] A bicistronic co-expression gene transfer body, the gene sequence of the gene transfer body is: SEQ ID No.15.
[0061] And, if Figure 4 As shown, the gene transfer body includes bovine nuclear matrix binding region MAR, artificially constructed combined promoter CAG, suppressor protein gene FSTN, internal ribosome entry site IRES, green Fluorescent protein AcGFP gene, Rabbit globin polyA signal region.
[0062] Moreover, the sequence of the nuclear matrix binding region MAR is obtained by performing PCR with the following primers:
[0063] MAR sequence before CAG:
[0064] CMAF1 sense strand, 5' to 3': SEQ ID No.9;
[0065] CMAR2 antisense strand, 5' t...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com