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Method using three-bacterium mixed fermentation to convert sorbose into 2-keto-L-gulonic acid

A technology of mixed fermentation and sorbose, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem that the associated factors cannot satisfy the growth of acid-producing bacteria and the ability of acid-producing bacteria to produce acid for a long time Keeping it in the best state, the second step mixed bacteria fermentation efficiency is difficult to be effectively improved and other problems, to achieve the effect of making up for the low fermentation efficiency, eliminating the low efficiency of acid production, and shortening the fermentation cycle

Active Publication Date: 2014-01-08
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Because the high-efficiency associated ability of an associated bacterium lasts for a short period of time, it is difficult to continuously provide sufficient effectors throughout the fermentation stage. Therefore, the acid-producing ability of acid-producing bacteria cannot be maintained at the best state for a long time during the fermentation process, making the second It is difficult to effectively improve the efficiency of two-step mixed bacteria fermentation
In addition, due to the lag of the test results and the complexity of the multi-stage expansion process of the seed liquid, the large tanks of the two-step mixed-bacteria fermentation often appear to be contaminated with bacteria and phages, and the phages kill the associated bacteria, making the associated factors unable to meet the growth of acid-producing bacteria. and fermented acid, resulting in a significant reduction in fermentation speed and even the occurrence of tanks, which seriously affected the production of gulonic acid and caused a large amount of energy consumption and material loss

Method used

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  • Method using three-bacterium mixed fermentation to convert sorbose into 2-keto-L-gulonic acid

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Experimental program
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Effect test

Embodiment 1

[0021] The strain A composed of Bacillus cereus and keto-cologronate and the strain B composed of Bacillus thuringiensis and keto-cologronate were respectively taken from the seed solution of the slant, respectively, with 5% (volume ratio) As the inoculum amount of the seed solution for three-stage continuous amplification, it was inoculated in different grades of seed culture medium (seed culture medium, peptone 0.3% by weight percentage, beef extract 0.3%, yeast extract 0.3%, corn steep liquor 0.3%) , MgSO 4 0.01%, FeSO 4 0.01%, L-sorbose 2%, pH 7.0, agar 2.5%, the balance is water; sterilize at 121°C for 20 minutes; set aside. ), cultured in shake flasks for 20 hours until the yield of guronic acid in different grades of fermentation broth was 8-10mg / ml, and transferred to the next grade;

[0022] After the above-mentioned seed liquid is expanded through three stages, get A seed liquid 2.4ml and B seed liquid 0.8ml respectively, altogether 3.2ml inserts and is equipped ...

Embodiment 2-4

[0024]

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Abstract

The invention belongs to the technical field of vitamin C biological fermentation, and particularly relates to a method using three-bacterium mixed fermentation to convert sorbose into 2-keto-L-gulonic acid which is in a second step of the vitamin C biological fermentation. Seed solution of manufacturing technique of the second step of the vitamin C biological fermentation is enlarged step by step, when in fermental cultivation, with total inoculation amount with volume percent of 15-20%, seed solution of A isolates and B isolates are introduced into a fermentation tank to be fermented and cultivated to a fermentation ending point (namely when the concentration of remained sorbose in fermentation liquor is smaller than or equal to 1mg / ml), and the purpose that the mixed bacteria fermentation is adopted for converting the sorbose to the 2-keto-L-gulonic is achieved. The A isolates is composed of companion fungus bacillus cereus / companion fungus bacillus megatherium and acid-forming bacteria ketogenic base ketogulonigenium vulgare. The B isolates is composed of companion fungus bacillus thuringiensis and acid-forming bacteria ketogenic base ketogulonigenium vulgare. According to the novel method using the three-bacterium mixed fermentation, respective advantages of the two kinds of the companion funguses are made full use, fermentation period can be shortened by 6-8 hours, energy consumption is reduced by 10-15%, and fermentation conversion percent is improved by 2-4%.

Description

technical field [0001] The invention belongs to the technical field of vitamin C biofermentation, and specifically relates to a method for transforming sorbose 2-keto-L-gulonic acid by using mixed bacteria in the second-step biofermentation of vitamin C. Background technique [0002] The industrial production of Vc two-step fermentation, the second step fermentation is the mixed fermentation of two kinds of bacteria to realize the biotransformation from L-sorbose to 2-keto-L-gulonic acid. The two types of bacteria include bacteria with acid production ability - common ketogenic cologne (commonly known as acid-producing bacteria or small bacteria) and associated bacteria that do not have the ability to transform acid production but can promote the growth and acid production of small bacteria (commonly known as large bacteria). Existing research results have shown that common keto-L-gulonic acid bacteria have weak subculture survival rate and 2-keto-L-gulonic acid production ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/60C12R1/07C12R1/085C12R1/11
Inventor 杨伟超徐慧满都拉张忠泽安海英胡昊李英杰韩利涛姜铭妍谌明徐静曹桂环
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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