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Method for extracting and purifying 6-gingerol from ginger

A technology of ginger extract and gingerol, applied in the field of natural medicine preparation, can solve the problems of complex composition, low separation efficiency, complicated operation and the like, and achieve the effects of simple preparation process, high separation efficiency and high separation purity

Active Publication Date: 2013-03-20
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The supercritical extraction method can obtain the volatile oil and gingerol of ginger at the same time. The components are relatively complex, and the gingerol content is not high, which is not conducive to the subsequent separation; in the HPLC separation step, pure methanol is used as the mobile phase, which has low separation efficiency and high cost , not suitable for large-scale application
[0007] To sum up, the existing preparation process steps are relatively long, the operation is cumbersome, and the product yield is not high. It is only suitable for small-scale preparation in the laboratory, and it is difficult to apply and large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 A method for extracting and purifying 6-gingerol from ginger, comprising the following steps:

[0030] (1) Extraction and concentration of gingerol:

[0031] 10 kg of sliced ​​ginger was placed in a small-scale multifunctional extraction and concentration tank (HL-300, Wuhan Huipeng Pharmaceutical Machinery Equipment Co., Ltd.), and the mass concentration was 80% ethanol at a mass-volume ratio of 1:15 (kg / L). Heating and reflux extraction at 60°C. The extraction time is 2 hours each time. Each batch of ginger is extracted twice. The extraction solution of each extraction flows into a small multi-functional extraction and concentration tank, and the ethanol is recovered under reduced pressure at a temperature of 60°C to obtain ginger. Extract.

[0032] ⑵ Gingerol Extraction:

[0033] The ginger extract was placed in a glass reaction kettle, extracted with ethyl acetate, and the extraction times were 3 times, and the extract was combined to obtain an extr...

Embodiment 2

[0042] Example 2 A method for extracting and purifying 6-gingerol from ginger, comprising the following steps:

[0043] (1) Extraction and concentration of gingerol:

[0044] 20 kg of sliced ​​ginger was placed in a small multifunctional extraction and concentration tank (HL-300, Wuhan Huipeng Pharmaceutical Machinery Equipment Co., Ltd.), and the mass concentration was 95% ethanol at a mass-volume ratio of 1:6 (kg / L) in the Heating and reflux extraction at 70°C. The extraction time is 2.5 hours each time. Each batch of ginger is extracted 4 times. The extraction solution of each extraction flows into a small multi-functional extraction and concentration tank, and the ethanol is recovered under reduced pressure at a temperature of 60°C to obtain ginger. Extract.

[0045] ⑵ Gingerol Extraction:

[0046] The ginger extract was placed in a glass reactor, extracted with ethyl acetate, and the extraction times were 4 times, and the extract was combined to obtain the extract...

Embodiment 3

[0055] Example 3 A method for extracting and purifying 6-gingerol from ginger, comprising the following steps:

[0056] (1) Extraction and concentration of gingerol:

[0057] 20 kg of sliced ​​ginger was placed in a small multifunctional extraction and concentration tank (HL-300, Wuhan Huipeng Pharmaceutical Machinery Equipment Co., Ltd.), and the mass concentration was 90% ethanol at a mass-to-liquid ratio of 1:8 (kg / L) in the Heating and reflux extraction at 80°C. The extraction time is 3 hours each time. Each batch of ginger is extracted 3 times. The extraction solution of each extraction flows into a small multi-functional extraction and concentration tank, and the ethanol is recovered under reduced pressure at a temperature of 60°C. Concentrate to obtain ginger extract.

[0058] ⑵ Gingerol Extraction:

[0059] The ginger extract was placed in a glass reactor, extracted with ethyl acetate, extracted twice, and combined to obtain the extract; the extract was recover...

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Abstract

The invention relates to a method for extracting and purifying 6-gingerol from ginger, which comprises the following steps: (1) extraction and concentration of gingerol: heating sliced ginger in ethanol under reflux, and concentrating to obtain a ginger extract; (2) leaching of gingerol: leaching the ginger extract to obtain a leaching solution, and recycling the extract under reduced pressure to obtain a gingerol crude extract; (3) silica gel column chromatography: dissolving the gingerol crude extract in ethyl acetate, adding silica gel, mixing, and after the solvent is volatilized, passing through a silica gel column by a dry process; after carrying out isocratic elution and thin-layer chromatography (TLC) detection, collecting the eluate part containing 6-gingerol, recycling the eluate under reduced pressure to obtain a 6-gingerol crude product; passing the 6-gingerol crude product through the silica gel column, and carrying out elution, detection, collection and solvent recycling to obtain the higher-purity 6-gingerol; and (4) purification by preparative HPLC (high performance liquid chromatography): dissolving the higher-purity 6-gingerol, purifying by preparative HPLC, carrying out isocratic elution, collecting the part with the maximum chromatogram peak, and drying by distillation to constant weight, thereby obtaining the high-purity 6-gingerol. The technique is simple and easy to operate; and the product has the advantages of high yield and good quality.

Description

technical field [0001] The invention relates to the technical field of natural medicine preparation methods, in particular to a method for extracting and purifying 6-gingerol from ginger. Background technique [0002] Ginger is the fresh rhizome of the Zingiberaceae Zingiberaceae plant Zingiber officinale. It is one of the commonly used Chinese medicinal materials in my country, and it is also one of the first batch of traditional Chinese medicines with the same origin of medicine and food announced by the Ministry of Health. Ginger has been traditionally used throughout the world in the treatment of a wide variety of ailments in humans, including arthritis, rheumatism, sprains, muscle pain, pain, sore throat, constipation, indigestion, vomiting, high blood pressure, Alzheimer's, fever, Infectious diseases and helminths etc. Ginger has a medicinal history of 2,500 years. It was first recorded in "Shen Nong's Materia Medica" in China, and it was listed as a middle grade. Th...

Claims

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Application Information

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IPC IPC(8): C07C49/255C07C45/78
Inventor 岳会兰邵赟梅丽娟陶燕铎
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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