Molecular identification method for distant hybridization progeny of Sesame indicum L. and Sesame radiatum

Abstract

Belonging to the field of biotechnologies, the invention discloses a molecular identification method for a distant hybridization progeny of Sesame indicum L. and Sesame radiatum. The method includes: extracting the genomic DNA of Sesame indicum L., 2n=26, the Sesame radiatum, 2n=64 and the hybridization progeny F1, conducting PCR amplification and gel electrophoresis detection, and determining whether the distant hybridization progeny F1 is true or false according to whether parental specific bands can be amplified simultaneously. The molecular identification method for a sesame distant hybridization progeny established in the invention solves the problems of long distant hybrid identification cycle, low accuracy and the like in the process of sesame distant hybridization breeding study, and fills the vacancy of sesame distant hybridization breeding technology in China. By means of the method, the sesame distant hybridization progeny F1 can be identified efficiently and rapidly, thus laying the material and technology bases for breeding of new disease-resistant sesame varieties.

Description

technical field [0001] The invention relates to a molecular identification method for plant hybrid offspring, in particular to a molecular identification method for distant hybrid offspring of cultivated sesame (2n=26) and wild species (Sesame radiatum, 2n=64), belonging to the field of biotechnology . Background technique [0002] Sesame (Sesame indicum L., 2n=26) is one of the oldest characteristic oil crops in the world. Sesame has been cultivated in my country for more than 3,000 years. Because of its high quality, it occupies an important position in international production and trade. However, due to the poor resistance to disease and water stains of sesame, it is easily affected by environmental conditions, and the stability of yield is not good. In the past two decades, the development of high-yield, stable-yield, disease-resistant and stress-resistant new varieties has always been a key task of sesame genetics and breeding in my country. [0003] Wild sesame spec...

AI Technical Summary

Problems solved by technology

However, due to the incompatibility of sesame distant hybridization, the progeny can hardly self-fertilize. At present, only the immature embryo rescue culture and tissue culture seedling transplanting methods can be used to obtain distant hybrid offspring.

In the identification of the offspring of distant hybrids, due to the limitation of research technology, only identification methods such as appearance and biology are used to judge the authenticity of the F1 generation of sesame distant hybrids

The study found that, unlike the seedlings, the tissue-cultured seedlings of sesame distant hybrids had no obvious differences in appearance, but the differences with their parents were significant.

Therefore, methods such as appearance and biology are used to identify the offspring of distant hybrids, and the reliability of the results is poor, and the identification period is long

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