Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lentinan calcium complex, and preparation method and application thereof

A technology of lentinan and complexes, applied in the fields of application, food preparation, food science, etc., can solve the problems of poor water solubility, low bioavailability, precipitation, etc., achieve high absorption rate, good water solubility, and simple and easy preparation method line effect

Inactive Publication Date: 2013-03-13
SOUTH CHINA UNIV OF TECH
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the common calcium supplement products on the market are mostly inorganic calcium, and its calcium source is absorbed in the form of ionized calcium. Calcium ions tend to react with other anions in the human gastrointestinal tract, resulting in precipitation, poor water solubility, and low bioavailability.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lentinan calcium complex, and preparation method and application thereof
  • Lentinan calcium complex, and preparation method and application thereof
  • Lentinan calcium complex, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The present invention is realized by the following steps in the specific implementation process:

[0022] (1) Prepare calcium chloride solution and sodium hydroxide solution: prepare 2mol / L calcium chloride solution with distilled water; prepare 2mol / L sodium hydroxide solution with distilled water;

[0023] (2) Weigh 100 mg of lentinan, dissolve it in 5 mL of deionized water, and add sodium hydroxide drop by drop to the solution until the pH value is 8. Under stirring, calcium chloride with a mass ratio of 5:1 to polysaccharide was added dropwise, continued stirring in a water bath at 60°C for 1 hour, and dialyzed in a dialysis bag with a molecular weight cut-off of 3500 Da for 2 days to cut off free calcium ions. After freeze-drying, lentinan-calcium complex L2-Ca80.2 mg was obtained.

[0024] (3) Take 2.0 mg of lentinan and lentinan calcium complex, mix them with KBr, grind them, and press them into tablets. Use Nicolet Nexus 5DXC FT-IR at 500-4000cm -1 Scanning wi...

Embodiment 2

[0027] The present invention also can be realized by the following steps in concrete implementation process:

[0028] (1) Prepare calcium chloride solution and sodium hydroxide solution: prepare 3mol / L calcium chloride solution with distilled water; prepare 3mol / L sodium hydroxide solution with distilled water;

[0029] (2) Accurately weigh 50 mg of lentinan, dissolve it in 5 mL of deionized water, and add sodium hydroxide drop by drop to the solution until the pH value is 8.5. Under stirring, drop calcium chloride with a polysaccharide mass ratio of about 4:1, continue stirring in a 70°C water bath for 90 minutes, and dialyze in a dialysis bag with a molecular weight cut-off of 8000Da for 3 days to remove free calcium ions. After freeze-drying, the lentinan-calcium complex was obtained, and the detection steps and results were basically the same as in Example 1.

Embodiment 3

[0031] The present invention also can be realized by the following steps in concrete implementation process:

[0032] (1) Prepare calcium chloride solution and sodium hydroxide solution: prepare 4mol / L calcium chloride solution with distilled water; prepare 4mol / L sodium hydroxide solution with distilled water;

[0033] (2) Accurately weigh 100 mg of lentinan, dissolve it in 30 mL of deionized water, and add sodium hydroxide drop by drop to the solution until the pH value is 9. Under stirring, add calcium chloride with a mass ratio of about 2:1 to the polysaccharide dropwise, continue to stir in a 75°C water bath for 80 minutes, and dialyze in a dialysis bag with a molecular weight cut-off of 1000Da for 4 days to remove free calcium ions. After freeze-drying, the lentinan-calcium complex was obtained, and the detection steps and results were basically the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a lentinan calcium complex, and a preparation method and application thereof. The method comprises the steps of: adding a calcium chloride solution to an aqueous solution of lentinan with stirring, at 40-80 DEG C, regulating a pH value to 7-10 with sodium hydroxide, and continuously stirring for 1 h for a complex reaction; removing free calcium ions by a dialysis method; and concentrating and freeze-drying the lentinan compound solution to obtain the lentinan calcium complex with calcium content of 5.2mg / g. Compared with a blank group, the lentinan calcium complex can obviously promote mouse macrophage RAW264.7 to release immune factors, such as a tumor necrosis factor TNF-alpha and a cell factor IL-6. The preparation method of the lentinan calcium complex provided by the invention is simple and easy for practice. Besides, as an organic calcium, the lentinan calcium complex can also promote calcium absorption and improve immunity.

Description

technical field [0001] The invention relates to the fields of biopharmaceutical engineering and health food, in particular to a lentinan calcium complex and a preparation method and application thereof. Background technique [0002] Lentinus edodes belongs to the Basidiomycetes Pleurotaceae fungus, and is one of the world's famous edible and medicinal fungi. It contains a variety of effective medicinal ingredients, and its main active ingredient, lentinan, is a polar macromolecular compound, mainly derived from the cell wall of the fungus. There are some intracellular polysaccharides. Studies have confirmed that lentinan has various biological activities such as anti-virus, anti-tumor, anti-radiation, anti-aging, anti-stress, anti-oxidation, and immunity-enhancing activities, among which anti-tumor, anti-oxidation, and immunity-enhancing activities have been studied more. [0003] Calcium is one of the most abundant mineral elements in the human body, and has extremely impo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04A61P3/14A23L1/09
Inventor 张学武闫慧丹
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com