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Quantum dot targeting probe for colorectal cancer tumor tissue identification and preparation method thereof

A tumor tissue and tumor targeting technology, applied in preparations for in vivo experiments, chemical instruments and methods, pharmaceutical formulations, etc., can solve problems such as poor stability, influence of coupling agents, and restrictions on popularization and use, and achieve stability High, fast combining speed, highly reproducible results

Inactive Publication Date: 2013-03-13
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is: in order to overcome the problems such as poor binding stability between existing polypeptide ligands and QDs and being affected by coupling agents, and limit their popularization and use in the biological field

Method used

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  • Quantum dot targeting probe for colorectal cancer tumor tissue identification and preparation method thereof
  • Quantum dot targeting probe for colorectal cancer tumor tissue identification and preparation method thereof
  • Quantum dot targeting probe for colorectal cancer tumor tissue identification and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The method of the present invention adopts the conventional solid-phase Fmoc method, that is, the monomer amino acid protected by Fmoc on the solid-phase resin is deprotected and the amino group is exposed, and a peptide bond is formed with the carboxyl group of the amino acid in the solution through a condensation reaction, thereby connecting the amino acid to On the resin, the peptide chain extends from the C-terminus to the N-terminus.

[0020] 1. Basic materials:

[0021] (1) Resin and connecting molecule: The resin selected by the solid phase Fmoc method is Rink Amide-ChemMatrix ? Resin. This resin has very good swelling properties, can make the condensation reaction between peptide chains better, and there is enough network space to meet the growing peptide chains. HBTU and HOBt are used as linking molecules to fix the peptide molecules on the resin.

[0022] (2) Monomer: The monomer used in the synthesis is a chemically modified α-amino acid.

[0023] 2. Reaction steps...

Embodiment 2

[0042] The polypeptide synthesis steps are the same as in Example 1. The synthetic peptide ligand sequence is as follows:

[0043] cyclo(1, 9)-CTPSPFSHCGP 10 G 2 H 7 .

Embodiment 3

[0045] The polypeptide synthesis steps are the same as in Example 1. The synthetic peptide ligand sequence is as follows:

[0046] cyclo(1, 9)-CTPSPFSHCGP 11 G 2 H 8

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Abstract

The invention discloses a method for preparing a quantum dot targeting probe (TCP-1-H6) for colorectal cancer tumor tissue identification and belongs to the field of nano biotechnology. A novel polypeptide ligand comprises histidine sequences (1) for combining with quantum dots, a sequence (2) for providing a polypeptide rigidity structure and a targeting sequence (3), wherein various sequences are combined through peptide bonds. The bifunctional ligand is simple in synthesis method, the N end is combined with the quantum dots through six histidine sequences, the stability of the ligand and the quantum dots is greatly improved, and the polypeptide ligand can serve as a nano fluorescence probe to be applied to colorectal cancer tumor marking.

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a quantum dot targeting probe for colon cancer tumor tissue recognition and a preparation method thereof. Background technique [0002] Quantum dots (QDs) are zero-dimensional semiconductor nanocrystals, approximately spherical, with a diameter of 1-12nm, which can be dispersed in water or organic solvents to form colloids. Since the size of QDs is close to or even smaller than the excitons (electron- hole pair) Bohr radius, the electrons and holes generated when excited are confined in a narrow three-dimensional space, thus exhibiting quantum effects. Compared with traditional organic dyes, QDs have more excellent spectral properties, such as wide excitation spectrum And continuous distribution, while the emission spectrum is symmetrically distributed with narrow width, adjustable color, and high photochemical stability, not easy to photolysis (Marcel BJ, Mario M, Peter G, et a...

Claims

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Application Information

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IPC IPC(8): A61K49/00C07K19/00
Inventor 王建浩王车礼蒋鹏举邱琳夏江
Owner CHANGZHOU UNIV
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