Real-time fluorescent quantitative PCR method used for detecting Mycobacterium tuberculosis, and primer, probe and kit thereof
A real-time fluorescence quantitative technology for Mycobacterium tuberculosis, which is applied in the field of biomedicine, can solve the problems that the specificity of detection results needs to be improved, the 16S gene is short, and the detection specificity is low, and achieves the effect of good application prospect and good detection specificity.
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[0028] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.
[0029] The following examples are only used to illustrate the present invention, but not to limit the scope of the present invention. The experimental method that does not indicate specific condition in the embodiment, generally according to conventional conditions, such as people such as Sambrook, molecular cloning: the condition described in the laboratory handbook (New York: Cold Spring Harbor Laboratory Press, 1989), or according to manufacturer's suggested conditions.
[0030] 1. Sample collection and processing, and sample DNA extraction
[0031] Sputum samples were collected from patients in accordance with clinical sample collection requirements, and the collected samples were stored at -20°C.
[0032] Thaw the sample on ice, take 2 mL of the sample, add 4 mL of 4% NaOH solution to the sample, mix well, and treat at room temperature ...
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