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Method for producing microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus

A technology of microbial polysaccharide and Paenibacillus, which is applied in the field of producing microbial polysaccharide fermentation liquid by using Paenibacillus colloid, achieves the effects of improving product grade, short fermentation time and eliminating equipment requirements

Active Publication Date: 2014-01-15
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the new application fields of its polysaccharides and the optimization of the process to increase the yield of polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for producing microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus is to inoculate Paenibacillus mucilaginosus ACCC10013 on a medium containing a carbon source and a nitrogen source for fermentation, including the following process steps:

[0027] A. Bacteria heat stimulation

[0028] Under sterile conditions, add the spores of the bacteria in the inclined plane of the test tube to sterile water, shake and wash the spores to make a spore suspension and place it in a sterile container, and place the sterile container containing the spore suspension in boiling water. Heat evenly, take out the aseptic container after 1 minute and cool down rapidly;

[0029] B. Fermentation

[0030] B1. Preparation of culture medium

[0031] Prepare the medium and sterilize it, and cool it to 30°C. The medium consists of the following components in mass percentage:

[0032] Starch 5%, sucrose 5%, ammonium sulfate 0.5%, dipotassium hydrogen phosphate...

Embodiment 2

[0041] In step A in this embodiment, after the aseptic container is evenly heated for 10 minutes, the aseptic container is taken out and rapidly cooled;

[0042] B. Fermentation

[0043] B1. Preparation of culture medium

[0044] Prepare the medium and sterilize it, and cool it to 30°C. The medium consists of the following components in mass percentage:

[0045] Starch 20%, sucrose 10%, ammonium sulfate 5%, dipotassium hydrogen phosphate 20%, magnesium sulfate 10%, ferric chloride 0.5%, calcium carbonate 5%, yeast extract 3%, the balance is sterile water, pH =7.5;

[0046] B2. Cultivate

[0047] Inoculate the strain treated in step A into the culture medium prepared in step B, the fermentation period is 60 hours, the culture temperature is 30°C, and the inoculation amount is the strain: the mass percentage of the culture medium after sterilization is 8%; the air volume is adjusted For: 0-4 hours ventilation ratio is 1: .5VVM, 4-12 hours ventilation ratio is 1: 0.7VVM, 12-2...

Embodiment 3

[0051] In the steps of this embodiment, place the aseptic container in which the spore suspension is housed and heat evenly in boiling water. After 8 minutes, the aseptic container is taken out and rapidly cooled;

[0052]B. Fermentation

[0053] B1. Preparation of culture medium

[0054] Prepare the medium and sterilize it, and cool it to 30°C. The medium consists of the following components in mass percentage:

[0055] Starch 15%, sucrose 8%, ammonium sulfate 3%, dipotassium hydrogen phosphate 15%, magnesium sulfate 8%, ferric chloride 0.3%, calcium carbonate 3%, yeast extract 1%, the balance is sterile water, pH =7.5;

[0056] B2. Cultivate

[0057] Inoculate the strain treated in step A into the culture medium prepared in step B, the fermentation period is 60 hours, the culture temperature is 30°C, and the inoculation amount is the strain: the mass percentage of the culture medium after sterilization is 8%; the air volume is adjusted It is: the ventilation ratio of 0-4...

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PUM

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Abstract

The present invention belongs to microbial polysaccharide fermentation, and particularly relates to a method for producing a microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus, wherein Paenibacillus mucilaginosus is inoculated in a culture medium containing a carbon source and a nitrogen source to carry out fermentation, and bacteria heat stimulation, addition of a surfactant during a fermentation process, and other manners are performed to optimize the production process. With the present invention, the problem of low yield of polysaccharides in the fermentation broth in the prior art is solved, and advantages of high polysaccharide yield in the fermentation broth, good bacterial growth, short fermentation period and the like are provided.

Description

technical field [0001] The invention belongs to the fermentation of microbial polysaccharides, in particular to a method for producing microbial polysaccharide fermentation liquid by Paenibacillus colloidus. Background technique [0002] The jelly-like Paenibacillus is a soil bacillus, and its colony is round, colorless, raised, sticky, transparent or translucent, with neat edges. In the past few decades, it has been developed and utilized as the main functional strain of microbial fertilizer, which has played an important role in the field of agricultural microbial preparations. In recent years, with the deepening of the research on Paenibacillus glialis, people began to pay attention to the research and application of its exopolysaccharide. However, the hotspots still focus on the analysis of the polysaccharide components produced by Bacillus colloidus and the ability of polysaccharides to decompose silicate minerals. There are still few studies on the new application fi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/01
Inventor 陈文杰章淑艳魏亚新李军秦艳梅赵从波韩涛王云鹏古述江刘丽娜
Owner 河北省微生物研究所有限公司
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