Application of composition of Chinese yarn polysaccharide and Chinese yarn protein
A technology of yam protein and yam polysaccharide, which is applied in the direction of drug combination, peptide/protein composition, medical preparations containing active ingredients, etc.
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Embodiment 1
[0015] a. Use the dried rhizome of the native yam as a raw material to remove plant impurities, weigh 10 kilograms and crush it, heat it with water twice the weight of the yam, at a temperature of 50°C, reflux and extract twice for 2 hours, filter and combine the extracts , The extract is filtered and concentrated to 50%;
[0016] b. Add 95% ethanol to the concentrate to make the alcohol content of the concentrate reach 30%, and remove the precipitate after precipitation occurs;
[0017] c. Continue to add 95% ethanol to the concentrated solution to make the alcohol content reach 90%. After the precipitate appears, let it stand for 3 hours. After the precipitate is fully precipitated, the supernatant is removed to collect the precipitate, and then dried and crushed , You can obtain a composition with a yellow-brown powdered yam polysaccharide content of 40-50% and a yam protein content of 30-40%.
Embodiment 2
[0019] a. Use the dried rhizome of the native yam as a raw material to remove plant impurities, weigh 10 kilograms and crush it, heat it with water 5 times the weight of the yam, at a temperature of 100 ℃, reflux and extract 3 times for 3 hours, filter and combine the extracts , Filter and concentrate to 50%;
[0020] b. Add 95% ethanol to the concentrate to make the alcohol content of the concentrate reach 30%, and remove the precipitate after precipitation occurs;
[0021] c. Continue to add 95% ethanol to the concentrated solution to make the alcohol content reach 90%. After the precipitate appears, let it stand for 6 hours. After the precipitate is fully precipitated, the supernatant is removed to collect the precipitate, and then dried and crushed , You can obtain a composition with a yellow-brown powdered yam polysaccharide content of 40-50% and a yam protein content of 30-40%.
Embodiment 3
[0023] a. Using the dried rhizome of the native yam as a raw material, removing plant impurities, weighing 10 kilograms, smashing, heating with water 3.5 times the weight of the yam, at a temperature of 80°C, refluxing and extracting 3 times for 2.5 hours, filtering, and combining the extracts. Filter and concentrate to 50%;
[0024] b. Add 95% ethanol to the concentrate to make the alcohol content of the concentrate reach 30%, and remove the precipitate after precipitation occurs;
[0025] c. Continue to add 95% ethanol to the concentrated solution to make the alcohol content reach 90%. After the precipitate appears, let it stand for 4.5 hours. After the precipitate is fully precipitated, the supernatant is removed to collect the precipitate, and then dried and crushed , You can obtain a composition with a yellow-brown powdered yam polysaccharide content of 40-50% and a yam protein content of 30-40%.
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