Method for producing propionic acid type mildewproof agent by utilizing microbial fermentation method, and application thereof

A propionic acid type antifungal agent and the technology of microbial fermentation method are applied in the field of producing propionic acid type feed antifungal agent by microbial fermentation method, which can solve the problems of large environmental impact, complicated technological process and high energy consumption, and achieves reduction of production cost, The effect of simple process flow and wide source of raw materials

Inactive Publication Date: 2013-02-13
BIOFORTE BIOTECHSHENZHEN CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Propionaldehyde oxidation process is mature and is currently the largest production method for propionic acid. However, this method has obvious disadvantages, such as complex process flow, various equipment, high requirements for equipment and pipeline materials; harsh reaction conditions and high energy consumption. , a big impact on the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Production of Antifungal Agent Containing Calcium Propionate by Liquid Fermentation

[0022] 1) Propionic acid bacteria fermentation

[0023] Bacteria: Propionibacterium freunderii ( Propionibacterium freudenreichii ), or its subspecies Propionibacterium seschei ( Propionibacterium shermanil );

[0024] Medium: 5%-10% glucose, 1%-3% yeast powder, 3%-5% CaCO 3 , 0.1%-0.3%K 2 HPO 4 , the others are water. Initial pH6.5;

[0025] Erlenmeyer flask culture: put a Cyclopropionic acid bacteria cell into the Erlenmeyer flask with 50ml medium, shake well, and anaerobically culture for 24-48 hours, as the seed solution;

[0026] 2) Fermentation conditions in the fermenter: connect the seed liquid to the culture medium of the fermenter, inoculum amount 2%, culture temperature 30°C, airtight anaerobic fermentation for 4-6 days. Propionic acid content reaches 10-30 g / L, or above.

[0027] Propionate produced during fermentation automatically interacts with CaCO in medium ...

Embodiment 2

[0031] Production of Antifungal Agent Containing Ammonium Propionate by Liquid Fermentation

[0032] A kind of microbial liquid fermentation method to produce propionic acid type antifungal agent, wherein each kilogram of propionic acid type antifungal agent contains: ammonium propionate and 550 grams of fermentation products of P. A total of 450 grams of 80 mesh corncob powder and silicon dioxide excipient, wherein 150 grams of corncob powder and 300 grams of silicon dioxide.

[0033] The preparation method that microbial liquid state fermentation method described in embodiment 2 produces ammonium propionate fungicide comprises the steps:

[0034] 1) The strains of Propionibacterium freunderi were prepared first. The formula of the seed liquid medium is as follows: glucose 2%, yeast powder 2%, K 2 HPO 4 0.1%, pH6.5, sterilized at 121°C for 20-30 minutes, cooled to 37°C, and set aside. Anaerobic culture was performed for 3 days at a culture temperature of 30°C.

[0035] ...

Embodiment 3

[0041] A microbial solid-state fermentation method is used to produce propionic acid type antifungal agent, wherein the components per kilogram of propionic acid type antifungal agent contain: 75 grams of calcium propionate, 925 grams of metabolites of Propionibacterium sherbyi and culture medium materials.

[0042] The preparation method that the microbial solid-state fermentation method described in embodiment 3 produces propionic acid type antifungal agent comprises the steps:

[0043] 1) The strains of Propionibacterium seschei were prepared first. The formula of the seed liquid medium is as follows: glucose 2%, yeast powder 2%, K 2 HPO 4 0.1%, pH6.5, sterilized at 121°C for 20-30 minutes, cooled to 37°C, and set aside. Anaerobic culture was performed for 3 days at a culture temperature of 30°C.

[0044] 2) Fermentation culture of Propionibacterium sejeuriani, the formula of the fermentation medium is as follows: bran 50%, corn steep liquor 0.4%, CaCO 3 10%, water 40%, ...

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PUM

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Abstract

The invention provides a method for producing a propionic acid type mildewproof agent by utilizing a microbial fermentation method. The method comprises the following steps: carrying out solid fermentation or liquid fermentation on any one propionibacterium selected from Propionibacterium freudenreichii, a Propionibacterium shermanii or other dairy propionibacteria to; enabling propionic acid produced in the fermentation process to react with CaCO3 in a solid culture medium or a liquid culture medium to obtain a calcium propionate, or enabling the propionic acid to directly react with ammonia water fed in a liquid fermenting process to generate an ammonium propionate; and drying a solid base material to produce a solid propionic acid type mildewproof agent comprising the calcium propionate, or concentrating a fermentation liquor to produce a liquid propionic acid type mildewproof agent comprising the ammonium propionate. Because the used strain belongs to the diary propionibacteria and is publicly known as a safe strain, the propionate produced by the method is not required to be separated from propionic acid bacteria, and can be directly used for preventing feeds and grains from being mildewed. Compared with existing chemical methods, the propionic acid type mildewproof agent provided by the invention has advantages of wide raw material source, mild production condition, simplicity in producing process, low energy consumption, environmental friendliness and the like.

Description

technical field [0001] The invention relates to the field of feed additive production, in particular to a method for producing a propionic acid type feed antifungal agent through microbial fermentation. Background technique [0002] Calcium propionate or ammonium propionate is the main component of propionic acid type antifungal agent. As a food additive, it is a safe and reliable antifungal agent for food and feed approved by the World Health Organization (WHO) and the United Nations Food and Agriculture Organization (FAO). It is widely used in food industry and animal husbandry, and it has a wide range of antibacterial effects on mold, yeast and bacteria. At present, the production methods of calcium propionate and ammonium propionate are mostly propionaldehyde oxidation, and then propionic acid is reacted with calcium hydroxide to generate calcium propionate, or reacted with ammonia acid and alkali to generate ammonium propionate. Propionaldehyde oxidation process is mat...

Claims

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Application Information

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IPC IPC(8): A23K1/16C12P7/52C12R1/01
Inventor 胡文锋胡锦艳庞旭朱剑锋
Owner BIOFORTE BIOTECHSHENZHEN CO LTD
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