High-sensitivity glucose electrochemical sensor and preparation method thereof

A high-sensitivity, electrochemical technology, applied in the field of high-sensitivity glucose electrochemical sensors and their preparation, can solve the problems of high detection limit and low sensitivity of electrochemical sensors, and achieves increased electron transfer, improved sensitivity, and good adsorption. Effect

Inactive Publication Date: 2013-01-30
CHANGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The problem to be solved by the present invention is: in the prior art, the electrochemical sensor has the defects of low sensitivity and too high detection limit

Method used

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  • High-sensitivity glucose electrochemical sensor and preparation method thereof
  • High-sensitivity glucose electrochemical sensor and preparation method thereof
  • High-sensitivity glucose electrochemical sensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1PdPt-CNFs

[0026] AWeigh 0.01g of solid carbon nanofibers (diameter 50~150nm, length 2~10μm), add 3.0ml 3.5mmol L -1 h 2 PtCl 6 ·3H 2 O and 3.0ml 3.0mmol L -1 K 2 PdCl 4 In the mixed solution, mix and ultrasonically stir for 30min.

[0027] B use 0.05mol L -1 The pH of the above solution was adjusted between 9.5 and 10 with sodium hydroxide, and stirred for 10 min.

[0028] C weighs 0.01g of NaBH 4 Dissolve in 25ml of deionized water, while stirring, use a rubber dropper to absorb the NaBH4 solution and slowly drop it into the adjusted pH mixed solution at a speed of 2 to 3 seconds / drop, and then stir for 2 hours. Then filtered, washed with deionized water, and dried in a 60-degree oven to obtain PdPt-CNFs.

[0029] figure 1 It is the XRD pattern of CNFs and PdPt-CNFs, and the upper pattern is the XRD pattern of PdPt-CNFs; the lower pattern is the XRD pattern of CNFs. It can be seen from the figure that there is a strong diffra...

Embodiment 2

[0030] Embodiment 2 Preparation of high-sensitivity glucose sensor

[0031] A glassy carbon electrode with a diameter of 3 mm was used as the base electrode, and Al at 0.3 and 0.5 μm 2 o 3 After the polishing powder is polished to a mirror surface, it is ultrasonically washed with 1:1 nitric acid, ethanol and deionized water for 3 minutes.

[0032] The prepared PdPt-CNFs were dispersed in DMF solvent at a concentration of 4 mg ml -1 , sonicate for 30 minutes, use a micro-sampler to take 2μl drop-coated on the surface of the glassy carbon electrode that has been treated above, and then drop-coat 2μl 6mg ml after drying at room temperature -1 The glucose oxidase was dried at room temperature, and the electrode was suspended above the saturated vapor pressure containing glutaraldehyde for 10 minutes, thereby preparing a high-sensitivity glucose electrochemical sensor.

Embodiment 3

[0033] Embodiment 3 detection experiment

[0034] The detection experiment is carried out on a CHI660D electrochemical workstation and a three-electrode system. The reference electrode is a saturated calomel electrode, the counter electrode is a platinum wire electrode, and the working electrode adopts the electrochemical sensor prepared in Example 2 of the present invention. Under optimized conditions , with 0.1mol L of pH=7.0 -1 The phosphate buffer solution is used as the bottom solution. At 0.6V, the time-current curves obtained by adding different concentrations of glucose are as follows: figure 1 As shown, the linear range of the glucose electrochemical sensor can be obtained (as figure 2 shown) is 2.5 μmol L -1 ~8.4mmolL -1 , with a sensitivity as high as 12.3 μAmmol -1 (174μAmmol -1 cm -2), the linear correlation is very good, R=0.997, and the detection limit is as low as 0.7μmol.

[0035] Compared with the prior art of the present invention, such as: Fabricati...

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Abstract

The invention provides a high-sensitivity glucose electrochemical sensor which is obtained by modifying the surface of a conventional electrode with a layer of a palladium-platinum alloy loaded carbon nanofiber composite, i.e., a sensitive film of PdPt-CNFs. A preparation method for the electrochemical sensor comprises the following steps: dispersing palladium-platinum alloy loaded carbon nanofibers in a solvent through ultrasonic dispersion to obtain a uniform dispersion liquid; dropwise coating the dispersion liquid on the surface of a conventional clean electrode; evaporating the solvent; then immobilizing glucose oxidase; and after air-drying at room temperature, carrying out cross-linking above a glutaraldehyde saturated vapor pressure liquid level so as to obtain the high-sensitivity glucose electrochemical sensor. According to the invention, the prepared nanocomposite has good biocompatibility and is beneficial for immobilization of biological enzyme, and the preparation method is simple and fast and has low cost; the electrochemical sensor prepared in the invention has high sensitivity and good stability and reproducibility, so the electrochemical sensor is expected to be widely used in fields like diagnosis of diabetes, clinical medicine and food process checking.

Description

technical field [0001] The invention belongs to the field of electrochemical sensors, in particular to a high-sensitivity electrochemical sensor for glucose and a preparation method thereof. Background technique [0002] With the rapid development of society and the improvement of people's living standards, the prevalence of diabetes has also increased. According to the report of the World Health Organization in 2011, there are currently 346 million people suffering from diabetes. Real-time and reliable detection of blood sugar concentration is the diagnosis. and an effective way to control diabetes. [0003] Electrochemical sensors are widely used in the electrochemical detection of living substances due to their advantages such as high sensitivity, good selectivity, simple and fast experiments, and low cost of required instruments and equipment. It is of great scientific significance and practical value to develop a high-sensitivity glucose sensor for detecting small chan...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 陈智栋丁妮妮张静王文昌
Owner CHANGZHOU UNIV
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