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Bacterial strain for producing fructosyl transferase and preparation method thereof

A technology of fructosyltransferase and bacterial strains, which is applied in the fields of fermentation engineering and microbial breeding, and can solve problems such as difficult to meet actual production, low activity, slow catalysis, etc.

Active Publication Date: 2013-01-30
QUANTUM HI TECH (CHINA) BIO CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen that naturally evolved microbial enzymes cannot guarantee effective catalysis under every condition, and often suffer from slow catalysis on unnatural substrates, low enzyme stability, low activity in the reaction phase, The reaction system in the compound enzyme is not optional (if glycosyltransferase) and other factors are limited, it is difficult to meet the needs of actual production

Method used

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  • Bacterial strain for producing fructosyl transferase and preparation method thereof
  • Bacterial strain for producing fructosyl transferase and preparation method thereof
  • Bacterial strain for producing fructosyl transferase and preparation method thereof

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Embodiment 1

[0045] (1) Preparation of the spore suspension of the starting strain

[0046] The embodiment is based on Aspeigillus oryzae BLB-21 (strain preservation number CMGCC No.2951; the application number is 200910018452.1, and the name is "A strain of Aspeigillus oryzae and its method for preparing high-purity galacto-oligosaccharides" published in ) as the initial strain. Under the experimental conditions of the present invention, the initial strain was subjected to three slant mediums (white granulated sugar 5.0%w / w, soybean meal 2.0%w / w, corn flour 0.3%w / w, agar 2.0%w / w; the slant used below The medium is the same as here) after activation culture, inoculate in 100ml fermentation medium (white sugar 5.0%w / w, soybean meal 2.0%w / w, corn flour 0.3%w / w and water, the same below), 30℃ The lower shake flask was cultured for 2 days and then filtered out to carry out the determination of the fructosyltransferase enzyme activity of the mycelia. The results of the measurement are shown in...

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Abstract

The invention discloses a bacterial strain for producing fructosyl transferase and a preparation method thereof. The bacterial strain disclosed herein is prepared by the following steps: firstly screening a bacterial strain by lithium chloride and UV mutagenesis, and then changing the property of fructosyl transferase in the bacterial strain by genome shuffling. The bacterial strain is named Aspergillus oryzae scut209, preserved in China Center for Type Culture Collection (CCTCC) in Wuhan, China, on April 10, 2012 with CCTCC Designation CCTCC No:M 2012106. The bacterial strain has high capability of producing fructosyl transferase and has high industrial application value, wherein the capability of producing fructosyl transferase reaches 34987U / g cell.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering and microbial breeding, in particular to a strain producing fructosyltransferase and a preparation method thereof. Background technique [0002] Fructooligosaccharides are functional oligosaccharides with a degree of polymerization of 2 to 9, which are formed by connecting fructosyl groups through β (2→1) glycosidic bonds, and belong to food raw materials. According to the structure, fructo-oligosaccharides can be divided into sucrose-fructose-oligosaccharides and fructo-fructo-oligosaccharides, in which sucrose-fructose-oligosaccharides are mainly composed of β(2→1) on the fructose (F) residue of sucrose A mixture of kestose (GF2), kestotetraose (GF3), kestopentaose (GF4) and kestose hexose (GF5) formed by glycosidic bonds linking 1 to 4 fructosyl groups (F). [0003] Fructosyltransferase can catalyze the production of fructooligosaccharides from sucrose. Its enzymatic reaction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N13/00C12N15/01C12R1/69
Inventor 张毅曾宪经林晓珊何小妮陈子健
Owner QUANTUM HI TECH (CHINA) BIO CO LTD
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