A method for composite microbial oil recovery in low permeability oilfields
A technology of compound microorganisms and compound microorganisms, which is applied in the fields of fluid production, earthwork drilling, wellbore/well components, etc., can solve the problems of large recoverable oil reserves, etc., to increase crude oil production, reduce surface and interfacial tension, and increase sweeping area effect
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Embodiment 1
[0030] A low-permeability oilfield composite microbial oil recovery method, characterized in that: the method includes the following steps: 1) The surfactant-producing bacteria and the polymer-producing bacteria are cultured in indoor shake flasks, and the primary and secondary seed tanks are amplified. And fermentation production in the production tank to obtain high-activity fermentation broth of surfactant-producing bacteria and fermentation broth of polymer-producing bacteria;
[0031] 2) Mix the fermentation broth of the surfactant-producing strain and the polymer-producing strain produced by the fermentation in step 1) at a volume ratio of 1:1 to obtain a mixed liquid, that is, a composite microorganism liquid;
[0032] 3) The composite microbial liquid obtained in step 2) is used as an oil-displacing agent to be directly injected into the oil layer through a water injection well to obtain crude oil from the production well.
Embodiment 2
[0034] 1. The method includes the following steps:
[0035] 1) Surfactant-producing bacteria and polymer-producing bacteria are cultured in indoor shake flasks, first-level and second-level seed tanks are amplified, and production tanks are fermented to obtain high-activity surfactant-producing bacteria. Fermentation broth of polymer-producing bacteria;
[0036] 2) Mix the fermentation broth of the surfactant-producing strain and the polymer-producing strain produced by fermentation in step 1) at a volume ratio of 1:1 to obtain a mixed liquid, namely: a composite microbial broth;
[0037] 3) The composite microbial liquid obtained in step 2) is used as an oil-displacing agent to be directly injected into the oil layer through a water injection well to obtain crude oil from the production well.
[0038] The surfactant-producing strain is Pseudomonas aeruginosa YM4 (Pseudomonas aeruginosa), and its metabolite is rhamnose surfactant. After three-stage fermentation, the surface tension of...
Embodiment 3
[0062] The invention was applied in the Yuanmao test area of Yanchang Petroleum Wayaobao Oil Production Plant. A microbial flooding test of 10 injection and 32 recovery was carried out. The injection method of water injection well was adopted. Since the field test, the injection pressure has dropped and the average injection pressure From 5.1MPa to 4.6Mpa, the pressure drop rate reaches 10%. The output of oil wells has increased steadily. A total of 17 oil wells in the test area have seen obvious oil increase effects. The average daily oil production of each well in the test well group has increased by more than 20%.
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