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Preparation method for chlorella antitumor polypeptide

A chlorella, anti-tumor technology, applied in the fields of anti-tumor drugs, food preparation, food science, etc., can solve the problems of large toxic and side effects, poor selectivity, drug resistance of tumor cells, etc.

Inactive Publication Date: 2013-01-02
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Malignant tumors are one of the diseases that seriously threaten human health and life. Although the existing anti-tumor drugs have certain curative effects on most tumors, there are still problems such as low treatment efficiency, poor selectivity, large toxic and side effects, and easy production of tumor cells. Drug resistance and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of chlorella anti-tumor polypeptide, the steps are as follows:

[0023] (1) Chlorella protein was extracted using a low-temperature ultrahigh-pressure continuous flow cell crusher: Mix chlorella powder and pure water at a mass ratio of 1:20 and stir for 50 minutes to obtain a mixed solution. Extract the chlorella protein from the above mixed solution at a temperature of 6°C and a pressure of 100 MPa, and obtain the crude extract as the next extraction solution for re-extraction. The number of extractions is 3 times, and the time for each extraction is 20min. Finally, the obtained solution was centrifuged at 4 °C and 6000 r / min for 30 min to remove the precipitate to obtain the protein supernatant, and then vacuum freeze-dried to obtain the chlorella protein powder.

[0024] (2) Based on the chlorella protein powder obtained in step (1), configure a chlorella protein solution with a concentration of 2%, and add trypsin for hydrolysis. The experime...

Embodiment 2

[0027] The preparation method of chlorella anti-tumor polypeptide, the steps are as follows:

[0028] (1) Chlorella protein was extracted using a low-temperature ultrahigh-pressure continuous flow cell crusher: Mix chlorella powder and pure water at a mass ratio of 1:30 and stir for 80 minutes to obtain a mixed solution. Extract the chlorella protein from the above mixed solution at a temperature of 8°C and a pressure of 150 MPa, and obtain the crude extract as the next extraction solution for re-extraction. The number of extractions is 6 times, and the time for each extraction is 50min. Finally, the obtained solution was centrifuged at 4 °C and 5000 r / min for 20 min to remove the precipitate to obtain the protein supernatant, and then quickly freeze-dried in vacuum to obtain the chlorella protein powder.

[0029] (2) Based on the chlorella protein powder obtained in step (1), configure a chlorella protein solution with a concentration of 2%, and add trypsin for hydrolysis. T...

Embodiment 3

[0031] The preparation method of chlorella anti-tumor polypeptide, the steps are as follows:

[0032] (1) Chlorella protein was extracted using a low-temperature ultra-high pressure continuous flow cell disruptor: Mix chlorella powder and pure water at a mass ratio of 1:35 and stir for 100 minutes to obtain a mixed solution. Extract the chlorella protein from the above mixed solution at a temperature of 9°C and a pressure of 200 MPa, and obtain the crude extract as the next extraction solution for re-extraction. The number of extractions is 2 times, and the time for each extraction is 10min. Finally, the obtained solution was centrifuged at 4 °C and 8000 r / min for 30 min to remove the precipitate to obtain the protein supernatant, and then quickly freeze-dried in vacuum to obtain the chlorella protein powder.

[0033] (2) Based on the chlorella protein powder obtained in step (1), configure a chlorella protein solution with a concentration of 2%, and add trypsin for hydrolysi...

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Abstract

The invention provides a preparation method for chlorella antitumor polypeptide. The preparation method is characterized in that chlorella protein is extracted by using a low temperature ultrahigh pressure continuous flow cell crusher, then is hydrolyzed by trypsin and finally filtered with an ultrafiltration centrifuge tube so as to obtain chlorella polypeptide with a molecular weight in ranges of 0 to 3 KD, 3 to 5 KD, 5 to 10 KD and more than 10 KD. The chlorella polypeptide prepared in the invention has antitumor activity, e.g., the chlorella polypeptide has inhibitory effects on in vitro growth of human hepatoma carcinoma cells HepG2 and inhibition rates of chlorella polypeptide with a concentration of 1 mg / mL and in ranges of 0 to 3 KD, 3 to 5 KD and 5 to 10 KD on in vitro growth of hepatoma carcinoma cells (HepG-2) respectively reach 7%, 14% and 10%. Therefore, the chlorella antitumor polypeptide prepared in the invention is beneficial for development and utilization of antitumor health food and medicinal products.

Description

technical field [0001] The invention relates to the field of chlorella deep processing and biotechnology, in particular to a preparation method of chlorella anti-tumor polypeptide. Background technique [0002] Chlorella pyenoidosa is a kind of universal single-celled green algae, which belongs to Chlorophyta and the genus Chlorella. It grows fast, is easy to cultivate, and has high application value. As a new type of health food and medicine, it has great potential. Chlorella has anti-tumor activity, increases immunity, detoxifies and protects the liver, and lowers blood pressure. Its crude protein content is high (about 50%) and its quality is good. It has become a very active and highly valued application field of chlorella. aspect. [0003] Bioactive peptides refer to those peptides with special physiological activity or functional properties. The vast majority of proteins are precursors of biologically active peptides with certain functional roles. There are amino a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61P35/00A23L1/29A23L33/00
Inventor 张学武王晓琴
Owner SOUTH CHINA UNIV OF TECH
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