Synthetic peptide and application thereof

A technique for synthesizing peptides and wound healing agents, applied in the field of biology, can solve the problems of natural antibacterial peptide content, activity, activity-dependent structure, production cost, etc., achieve significant wound healing effect, realize the effect of low dosage of production and use

Inactive Publication Date: 2014-07-23
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are many defects in natural antimicrobial peptides in terms of content, activity, activity-dependent structure, production cost, etc., which have become the biggest obstacle restricting antimicrobial peptides from entering practical applications.

Method used

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  • Synthetic peptide and application thereof
  • Synthetic peptide and application thereof
  • Synthetic peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation and purity detection of embodiment 1 synthetic peptide

[0028] In this example, the English parameters involved are well known to those skilled in the field of analytical chemistry, and reference can be made to "Handbook of Analytical Chemistry (6) Liquid Chromatographic Analysis" edited by Zhang Yukui. In addition, TFA refers to trifluoroacetic acid, Acetonitrile refers to acetonitrile, and HCOOH formic acid; the concentration of the mobile phase involved is the volume concentration.

[0029] a chemical synthesis

[0030] The synthetic peptide was chemically synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd., purified and confirmed by HPLC.

[0031] Two HPLC analysis of the high-affinity binding polypeptide

[0032] 1 The chromatographic conditions are as follows:

[0033] Measurement: Peak Area;

[0034] Run Time: 30min;

[0035] Calculation Type: Percent;

[0036] Wavelength: 220nm;

[0037] Flow rate: 1.0ml / min;

[0038] ...

Embodiment 2

[0047] The antibacterial activity of embodiment 2 synthetic peptides

[0048] The following strains were purchased from China Institute for the Control of Biological Products.

[0049] The antibacterial activity of antimicrobial peptides was determined according to the following steps:

[0050] LfcinB4-9 was used as a control antimicrobial peptide. Use distilled water to prepare the antimicrobial peptide into a 2560 μg / mL stock solution in a polypropylene centrifuge tube, then dilute it with an equal amount of sterile 0.02% acetic acid (containing 0.4% BSA by volume fraction), and then use a volume fraction of 0.01% acetic acid (containing 0.2% BSA solution by volume fraction) is carrying out a series of double dilutions to the solution after equivalent dilution to obtain a mass concentration of 1280, 640, 320-2.5 μg / mL (successive double dilution ) serial dilutions. Store at 4°C for later use.

[0051] Cultivate the bacteria to be tested on a sterilized broth agar plate o...

Embodiment 3

[0056] Example 3 Application of Synthetic Peptides in Promoting Wound Healing

[0057] 1 Establishment of diabetic mouse model

[0058] Select 8-week-old Kunming rats, fast for 12 hours, and inject 1% streptozotocin solution intraperitoneally at a dose of 120 mg / kg, and fast for 12 hours on the 3rd and 7th days after the injection, respectively. The blood glucose concentration of fasting mice was measured by venous blood collection, and those whose blood glucose concentration exceeded 11.1mmol / L for two consecutive times were regarded as successful establishment of the mouse diabetes model. The mice whose blood glucose concentration reached the requirement were used to establish the trauma model.

[0059] 2 Establishment of mouse trauma model

[0060] Intraperitoneal injection of 7g / L pentobarbital sodium anesthetized mice with 0.7g / kg dose, depilated with 8% sodium sulfide, and wiped with warm water for 2-3 minutes so as to wash off the coat taken off at this position. In ...

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Abstract

The invention relates to the technical field of biology, in particular to a synthetic peptide containing an amino acid sequence shown as SEQ ID NO: 1. The invention also relates to application of the synthetic peptide in preparation of anti-infective drugs and wound healing drugs. The synthetic peptide is a hexapeptide formed by only six amino acids and is synthesized by a chemical method, so the production cost is reduced accordingly; compared with other complicated antibacterial peptides or growth factor peptide preparations and the like, the synthetic peptide is easier to produce and is suitable for being widely used in clinic; and the synthetic peptide provided by the invention has multiple activities, high antibacterial activity, a significant role in promoting wound healing, low dose, one-time daily application and obvious efficacies.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to synthetic polypeptides and applications thereof. Background technique [0002] Since the invention of antibiotics, human beings have made great achievements in the control and treatment of microbial infections, but with the continuous use of antibiotics, microbial resistance has become a major problem in infection control, so that some microbial bacteria have no control kill. first-line drug. As in clinical drug therapy, vancomycin-resistant staphylococci. Enterococcus and other Gram-negative bacterial infections are currently clinical problems worldwide. [0003] Antimicrobial peptides are small-molecule polypeptide substances with broad-spectrum antibacterial activity, effective on prokaryotes, and harmless to eukaryotes. Antimicrobial peptides are a class of peptides produced in the biological defense system against exogenous pathogens, and are an important part of biological i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61P31/04A61P17/02
Inventor 沈克飞王孝友杨睿徐登峰谷山林付利芝李大军葛良鹏
Owner CHONGQING ACAD OF ANIMAL SCI
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