A kind of high water retention microbial culture medium and preparation method thereof

Abstract

The invention discloses a microbial culture medium with high water retention and a preparation method thereof. It includes upper and lower layers, the lower layer is a resin gel layer containing superabsorbent resin, and the upper layer is an ordinary agar medium layer covered on the resin gel layer; or only one layer is a resin gel layer containing superabsorbent resin. In the present invention, superabsorbent resin is applied to the modification of microbial culture medium, and by optimizing the preparation process of the culture medium, the obtained culture medium improves its gel moisture and is easy to volatilize compared with ordinary culture medium; condensed water accumulates on the wall of the culture dish It is easy to cause contamination between colonies; freezing is easy to produce crystallization or cracking, which is not conducive to the shortcomings of strain passage and long-term storage, and reduces the cost of the product. It solves the inherent shortcomings of agar medium and the problem of medium loss during microbial collection. In addition to the same microbial growth and biocompatibility as ordinary media, the medium also has water absorption and water retention, as well as suitable gel strength and transparency.

Description

technical field [0001] The invention relates to a microbial culture medium and a preparation method thereof, in particular to a microbial culture medium with high water retention and a preparation method thereof. Background technique [0002] Since the German bacteriologist Koch first used gelatin as a solid medium coagulant for bacterial streaking, the coagulant has played an important role in bacterial culture, isolation, and identification. Subsequently, each hydrophilic coagulant was discovered one after another, and after being dissolved in water, it swelled to form a viscous solution or gel, and was used in microbial culture medium. At present, most of the bacterial culture medium in the prior art is based on agar, and some nutrients required for bacterial growth are added, and then the bacteria are cultivated, observed and measured in a sterile laboratory according to strict aseptic operating procedures. Agar is the most widely used coagulant, but there are still pro...

AI Technical Summary

Problems solved by technology

Agar is the most widely used coagulant, but there are still problems: 1) The moisture in the gel is easy to volatilize; 2) The condensed water accumulates on the wall of the petri dish and easily causes contamination between colonies; 3) Freezing is prone to crystallization or cracking, which is not conducive to Strain subculture and long-term preservation; 4) Overexploitation leads to high prices

Due to the need to use a large amount of organic solvents in the reverse phase suspension polymerization method, the main disadvantages are: the complexity of the synthesis process, the increase in cost, and the pollution to the environment

During the implementation of the natural sedimentation method of the petri dish, the petri dish needs to be exposed to the air, but because the moisture in the petri dish will gradually lose with the prolongation of the opening time, it will affect the growth of microorganisms settled on the medium. Reduce the number of microorganisms that can grow, and ultimately affect the accuracy of experimental data

During the implementation of the automatic collection method using the sampler, due to the effect of the air flow, the water on the medium will also be taken away, and eventually the water in the culture dish will be gradually lost, which will eventually affect the accuracy of the experimental data

[0007] In order to solve the problem of medium water loss during the collection and cultivation of microorganisms and prolong the cultivation time of microorganisms, we have developed a super absorbent resin for microbial culture medium

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