Method for identifying propolis and poplar glue by detecting chlorophyll content

A technology for chlorophyll content and chlorophyll, which is applied in the preparation of test samples and the measurement of color/spectral properties, etc., can solve the problems of expensive instruments, inability to distinguish between propolis and poplar gum, and high price. The effect of easy-to-obtain samples and equipment, and simple detection methods

Inactive Publication Date: 2012-11-14
杨茂森
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Propolis and poplar gum are very similar in sensory properties, and the chemical composition of total flavonoids is almost the same. According to the current domestic standards on propolis, it is difficult to distinguish between the two
For example: 1. In NY5136-2002 pollution-free food propolis released by the Ministry of Agriculture of the People's Republic of China, the total flavonoid content in propolis is determined by high performance liquid chromatography. The instrument is expensive and the process is complicated, and eight flavonoids galangin, rutin, Standard samples of quercetin, myricetin, camphenol, apigenin, pinenin, and coyne are expensive, and domestic purchases are not complete
Moreover, poplar gum also contains the aforementioned eight flavonoids, but their proportions are different, so it is impossible to fundamentally distinguish propolis from poplar gum
[0005] 2. In the GB / T 24283-2009 propolis issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, use ultraviolet spectrophotometry and use rutin as a reference substance to determine The content of total flavonoids in propolis, the cost of detection equipment and reference substances are relatively high, and the total flavonoids in poplar gum with rutin as the control are the same as the total flavonoids in propolis, and it is also impossible to distinguish propolis from poplar gum
[0006]3. In NY5136-2002 and GB / T 24283-2009, the ethanol extract content and oxidation time are measured, and poplar gum can also meet the requirements under the same conditions. These two detection indicators cannot distinguish between propolis and poplar gum

Method used

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  • Method for identifying propolis and poplar glue by detecting chlorophyll content
  • Method for identifying propolis and poplar glue by detecting chlorophyll content
  • Method for identifying propolis and poplar glue by detecting chlorophyll content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Principle of the method: extract the chlorophyll in the sample with acetone, layered extraction with ether, wash and purify with sodium sulfate solution, and quantify by colorimetry at 642nm and 660nm wavelengths.

[0026] Reagent: ethanol.

[0027] equipment:

[0028] Porous electronic constant temperature water bath, spectrophotometer, Erlenmeyer flask with stopper, 250ml, porcelain evaporating dish, glass mortar, 150ml, G 3 Glass sand core funnel, separatory funnel, 250ml, brown volumetric flask, 50ml, pipette, 10ml, 20ml.

[0029] 1. Analysis steps

[0030] 1.1. Processing and extraction of propolis: Take 5g of propolis sample, first freeze it at -5°C for 24h, then crush it and weigh it accurately; put it in a glass mortar, add 5g of calcium carbonate and 5g of quartz sand, and slowly grind it into For very fine powder, add 10ml of ethanol and continue to grind into a paste; transfer all the grinds to a 250ml conical flask with a stopper, wash the mortar with eth...

Embodiment 2

[0062] The principle of the method, reagents, instruments and equipment are the same as those in Example 1.

[0063] 1. Analysis steps

[0064] 1.1. Processing and extraction of propolis: take 5g of propolis sample, first freeze it at -10°C for 48h, then crush it and weigh it accurately; put it in a glass mortar, add 5g of calcium carbonate and 5g of quartz sand, and slowly grind it into For very fine powder, add 10ml of ethanol and continue to grind into a paste; transfer all the grinds to a 250ml conical flask with a stopper, wash the mortar with ethanol to make the total volume of ethanol reach 100ml, seal it tightly, soak in cold, Shake frequently within the first 10 hours, then let it stand for 48 hours, and use G 3 Suction filtration through a glass funnel, wash the Erlenmeyer flask repeatedly with acetone aqueous solution (85%) until the funnel is drained; pour the residue in the funnel back into the mortar and grind with acetone aqueous solution for 5 minutes, then tr...

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Abstract

The invention provides a method for identifying propolis and poplar glue by detecting chlorophyll content. Chlorophyll in a sample is extracted by using acetone, layered extraction is performed by using ether, the extract is cleaned and purified by using sodium sulfate solution, and colorimetry is performed under certain wavelength at two places to identify. The chlorophyll in the propolis or the poplar glue which is manufactured from poplar bark or poplar buds by using an organic solvent with very low polarity to performing extraction and separation, the chlorophyll content is quantitatively detected by spectrophotometry, the peak value and the average value of the chlorophyll content in the propolis and the poplar glue are calculated respectively, the safety limit value of the chlorophyll content in the propolis is set to be 15mg / 100g, and the propolis and the poplar glue can be accurately identified by compared detection. The detection method is simple and convenient to perform, the detected sample and equipment are simple and available, and the detection accuracy is high.

Description

technical field [0001] The invention relates to the technical field of identification of propolis and poplar gum, in particular to a method for identifying propolis and poplar gum through content detection of chlorophyll content. Background technique [0002] Propolis has many biological characteristics such as lowering blood fat, lowering blood sugar, improving human immunity, enhancing body resistance, anti-oxidation, anti-tumor, and preventing infection. It is widely used in human health care and disease treatment. Hotspots in the research and development of foreign bee products. [0003] Propolis is a fragrant solid made from the resin secreted by Western honey bees from the broken parts of plant buds, leaves or bark and mixed with bee mandibular gland secretions and beeswax. It is spread by bees on the gaps, frame ears, etc. of the beehive, and collected manually. bee products with natural properties. The annual output of natural propolis in my country is about 400 to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/34
Inventor 杨茂森刘泊王鑫
Owner 杨茂森
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