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Centrifugal microfluidic serum separation chip and preparation method thereof

A microfluidic chip and serum separation technology, which is applied in clinical diagnosis, health quarantine, and biomedical fields, can solve the problems of micro-full analysis systems, large centrifuge equipment, blood sample loss, etc., and reduce the chance of cross-contamination of blood samples , fast serum separation speed, simple and compact structure

Inactive Publication Date: 2012-11-14
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most hospitals and laboratories still use large centrifuges to separate serum and blood cells. This method requires blood samples in milliliters to be drawn from patients, but the actual samples used for testing are only a few microliters, resulting in a lot of waste of samples. ; Moreover, such centrifugal equipment has a large volume and complex mechanical structure. The serum separation process and detection are carried out separately and independently, which may easily cause loss and contamination of blood samples. Therefore, it is necessary to develop a new micro-separation technology

Method used

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  • Centrifugal microfluidic serum separation chip and preparation method thereof
  • Centrifugal microfluidic serum separation chip and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A polymethyl methacrylate (PMMA) chip was prepared by a CNC micromachining system, with a thickness of 3 mm and a diameter of 120 mm, and a central hole with a diameter of 20 mm was punched in the middle. Design the microstructure and microchannel graphics of the centrifugal microfluidic serum separation chip with computer-aided design drawing software (CAD), and then prepare various microchannels, microgrooves, Microwells, micropools, microareas, forming chip microstructures and microchannel systems, including microinjection areas, quantitative areas, waste liquid pools, quartz capillary grooves, split pools, serum areas, vents, and microfluidic flow channels . The PMMA chip and the blank PMMA chip engraved with microstructures and microchannels were washed with ethanol, deionized water, and ethanol in sequence, and then dried naturally. Put a quartz capillary with an outer diameter of 375 μm and an inner diameter of 25 μm into the groove of the quartz capillary, and ...

Embodiment 2

[0030] A polycarbonate (PC) chip was prepared by a CNC micromachining system, with a thickness of 5mm and a diameter of 120mm, and a central hole with a diameter of 30mm was punched in the middle. Use computer-aided design drawing software (CAD) to design centrifugal microfluidic serum separation chip graphics, and then prepare various microchannels, microgrooves, micropores, micropools, micro Area, forming the microstructure and microchannel system of the chip, including a micro-injection area, a quantitative area, a waste liquid pool, a quartz capillary groove, a split pool, a serum area, a vent, and a microfluidic flow channel. Wash the PC chip with the microstructure and microchannel and the blank PC chip sequentially with ethanol, deionized water, and ethanol, and then dry it naturally. Put a quartz capillary with an outer diameter of 375 μm and an inner diameter of 100 μm into the groove of the quartz capillary, fix and seal with epoxy glue. Align and press the PC chip ...

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Abstract

The invention relates to a centrifugal microfluidic serum separation chip and a preparation method thereof. The centrifugal microfluidic serum separation chip is a wafer-shaped microfluidic chip with a micro-structure and a micro-channel, and consists of a chip engraved with a micro-structure and a micro-channel and a blank chip. The centrifugal microfluidic serum separation chip is fixed on a rotation platform of a centrifugal machine, the separation operation of serum and erythrocyte in a micro-upgraded blood sample can be realized by the synergetic process of the micro-structure and the micro-channel on the chip, and the serum for analysis and detection can be acquired. The centrifugal microfluidic serum separation chip is applicable in related application fields, such as biomedicine, clinical diagnosis, health quarantine and the like.

Description

technical field [0001] The invention relates to a centrifugal microfluidic serum separation chip and a preparation method thereof, which are mainly used in multiple fields such as biomedicine, clinical diagnosis, and health quarantine. Background technique [0002] Blood testing is the most widely used sample testing in biomedicine, clinical diagnosis, and health and quarantine diagnosis, but the presence of red blood cells has a great interference on the spectral analysis in blood testing, so how to quickly and easily separate serum from whole blood has always been an issue. Hot and difficult issues in blood testing research. [0003] At present, most hospitals and laboratories still use large centrifuges to separate serum and blood cells. This method requires blood samples in milliliters to be drawn from patients, but the actual samples used for testing are only a few microliters, resulting in a lot of waste of samples. ; Moreover, such centrifugal equipment has a large v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00B01D17/038G01N1/34
Inventor 王晓东沙俊
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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