Method for culturing selenium-enriched cordyceps militaris

Abstract

The invention provides a method for culturing selenium-enriched cordyceps militaris. The method comprises the steps of (1) domestication of tolerance of cordyceps sinensis strains of selenium, (2) preparation and inoculation of a selenium-enriched culture medium and (3) selenium-enriched culture of fruiting bodies. The method has the following beneficial effects: the selenium-enriched cordyceps sinensis culture effect can be improved, that is, the enrichment factor in the prior art is less than 1 and is improved to be above 4.85; the content of total selenium in the fruiting bodies obtained through culture can reach 4-100mg / kg; the organic selenium in the selenium forms is above 90%; and the enrichment factor and the absorption and transformation efficiency are greatly improved.

Description

technical field [0001] The invention relates to a method for cultivating selenium-enriched Cordyceps sinensis, which belongs to the technical field of production of functional health care products. Background technique [0002] Selenium is an essential trace element for the human body and is closely related to human health. Selenium has various pharmacological effects such as anti-cancer, heart protection, liver necrosis, myopia and cataract prevention, detoxification, immunity improvement, anti-aging and enhancement of reproductive function. According to incomplete statistics, there are more than 40 countries and regions lacking selenium in the world, and 72% of the regions in my country are selenium-deficient regions. Therefore, it is being sought and developed for selenium-enriched products to ensure that the human body absorbs sufficient selenium. Scientists pay attention. [0003] Cordyceps militaris, also known as Cordyceps militaris, belongs to the Ascomycetes and the...

AI Technical Summary

Problems solved by technology

At present, there are some patents for the cultivation and production of artificial selenium-enriched Cordyceps militaris, "Cultivation method of selenium-enriched Cordyceps militaris (patent application number 201010252973.6)", "a cultivation method of selenium-enriched Cordyceps militaris (patent application number 200710010750.7)", "Selenium-enriched Cordyceps militaris artificial cultivation method and application of fruiting bodies (patent application number 200610046673.6)", "Selenium-enriched artificial cultivation method of militaris militaris" (patent application number 93117344.2) and other technical schemes are to carry out selenium-enriched strengthening of militaris militaris, But what above-mentioned technical scheme adopts all is to add inorganic selenite in the culture medium or spray the selenium fertilizer nutrient solution containing selenite, adopt selenite as the source of selenium element Cordyceps difficult to absorb, utilization rate is not high Therefore, the enrichment coefficient of selenium in the Cordyceps cultured by the above method is not high. The highest selenium content in the fruit body of Cordyceps militaris cultivated in these technical schemes is 3.83-81.95 μg / g, and the corresponding selenium concentration in the cultivation material is 1-32 mg / L, and the proportion of inorganic selenium in the obtained Cordyceps fruiting body is relatively large, so it is difficult to improve the nutritional and medicinal value of Cordyceps

"A method for cultivating selenium-enriched Cordyceps militaris" (patent application number 201010621575.7), "Cultivation method for selenium-rich Cordyceps militaris female species" (patent application number 201110279521.1) and other technical solutions improve the selenium source and use organic selenium yeast as The selenium source of the cultivation material, but because the cell wall of organic selenium yeast is too thick, Cordyceps can't effectively absorb and utilize selenium, resulting in low selenium utilization rate and low selenium enrichment coefficient. In the above technical scheme, selenium yeast is used to make the selenium concentration of the medium 50 mg / L, and the selenium content of the cultivated selenium-enriched Cordyceps militaris is only 35-50 mg / L

Moreover, the cost of organic selenium yeast is relatively high, and if the wall-breaking treatment is relatively complicated, and the cost is also high, it is not suitable for cultivating selenium-enriched Cordyceps as a selenium source