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Method for distinguishing soil N2O microorganism emission source

A technology of microorganisms and emission sources, applied in the field of soil microbial molecular ecology, can solve the problems of concentration drop, inability to completely inhibit soil nitrification, and result deviation, and achieve the effect of reducing disturbance

Active Publication Date: 2012-10-31
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

Due to the soil microbes on C during the cultivation process 2 h 2 The decomposition of will result in C 2 h 2 The concentration keeps decreasing, so that the nitrification of the soil cannot be completely inhibited, which makes the experimental results biased

Method used

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  • Method for distinguishing soil N2O microorganism emission source
  • Method for distinguishing soil N2O microorganism emission source

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Experimental program
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Embodiment

[0018] The experiment was carried out in Shilihe Town, Sujiatun District, Shenyang City, Liaoning Province. The soil was brown loam and the crops were soybeans. The N fertilizer is urea, which is fertilized once when planting, and the fertilization rate is 60kg N / ha. Soil samples were collected during the six developmental stages of the soybean growing season, namely the 19th, 35th, 52nd, 66th, 99th, and 115th days after planting. , branching stage, flowering stage, drum grain stage, mature stage. Each sampling time is from 9:00 am to 11:00 am. The specific method is: use a wooden block to place the ring knife on top of the ring knife, use a hammer to knock the ring knife into the soil until the upper edge of the ring knife is flush with the soil, then carefully dig the ring knife out of the soil with a shovel, and use a piece of iron Cut the soil at the lower edge of the ring knife flat, wrap the lower edge of the ring knife with a layer of thin plastic bag, and then tie it...

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Abstract

The invention relates to soil microbial molecular ecology and particularly relates to a method for distinguishing a soil N2O emission source. The method comprises the following steps of: after treating soil to be analyzed, determining the isotope delta 15N value of N2O emitted by the soil and the copy number of related microbial genes in the process that the soil produces the N2O, and judging that functional microbial groups with correlation test p <0.05 between gene abundance and delta 15N-N2O abundance value are microbial groups which play an important role in soil N2O emission. The method is applicable to the distinguishing of the microbial groups with N2O emission function in certain soil.

Description

technical field [0001] The invention relates to soil microbial molecular ecology, specifically a method for distinguishing soil N 2 O Method for Microbiological Emission Sources. Background technique [0002] Nitrous oxide (N 2 O) is one of the three important greenhouse gases, and its warming potential is that of CO 2 298 times (on a centennial time scale), and is also involved in the destruction of the ozone layer. Soils are considered to be the main N in terrestrial ecological processes 2 O emission sources, soil emission N 2 The distinction of O processes has always been a thorny problem. N produced in the soil 2 There are many processes of O, mainly involving nitrification, denitrification, denitrification of nitrifying bacteria, etc., and these processes may occur simultaneously in the soil. In addition, the soil produces N 2 The process of O is affected by many factors, such as temperature, moisture content, pH, available nitrogen, etc. The interaction and int...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04G01N27/62
Inventor 夏宗伟徐慧陈冠雄董丹孔双韩斯琴
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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