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Silk fibroin porous support made from polyvinyl alcohol, and preparation method and application thereof

A technology of silk fibroin and polyvinyl alcohol, which is used in inactive components of polymer compounds, medical science, prostheses, etc. It can solve the problem of restricting the rapid adsorption and growth and differentiation of surrounding tissue cells, affecting the sustained release of drugs, and the hydrophobicity of the material surface. Advanced problems, to achieve the effect of easy large-scale production and application, excellent degradation rate, and simple preparation process

Active Publication Date: 2012-10-24
SUZHOU SIMEITE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition, when silk fibroin is used for tissue engineering and tissue repair, the surface hydrophobicity of the material is too high, which limits the rapid adsorption and growth and differentiation of surrounding tissue cells.
The same reason makes its application in drug sustained-release agents have non-specific irreversible adsorption to some drug molecules, especially molecules containing a large number of hydrophobic groups, thus affecting the sustained release of drugs.

Method used

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  • Silk fibroin porous support made from polyvinyl alcohol, and preparation method and application thereof
  • Silk fibroin porous support made from polyvinyl alcohol, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Purification of silk fibroin

[0051] Put 10 grams of thick silk (thick silk for textile industry purchased from Jiangsu Suzhou Soho Group) in 2 liters of 0.02M sodium carbonate solution and boil for 30 minutes to remove the sericin wrapped on the silk surface (residual sericin can easily cause immune reactions ). The degummed silk was dried in the air and soaked in 50 milliliters of 9.3M lithium bromide solution. After heating at 60°C for 4 hours, a concentrated silk fibroin solution was obtained. Finally, the lithium bromide in the solution was removed by dialysis to obtain a concentration of about 8% ( w / v) silk fibroin aqueous solution. Silk fibroin solution can be stored at 4°C for 1-2 months.

[0052] 2. Preparation of silk fibroin porous sponge-like scaffold made of polyvinyl alcohol

[0053] 1) Dilute the silk fibroin solution to a concentration of 5% (w / v);

[0054] 2) Add 5% (w / v) polyvinyl alcohol (molecular weight 30,000-70,000) solution to the silk...

Embodiment 2

[0061] 1. Purification of silk fibroin

[0062] With embodiment 1.

[0063] 2. Preparation of silk fibroin porous sponge-like scaffold made of polyvinyl alcohol

[0064] 1) Dilute the silk fibroin solution to a concentration of 8% (w / v);

[0065] 2) Add 5% (w / v) polyvinyl alcohol solution to the silk fibroin aqueous solution and mix well, the weight ratio of the silk fibroin aqueous solution to the polyvinyl alcohol solution is 1:5;

[0066] 3) Transfer the blend of silk fibroin and polyvinyl alcohol into a dialysis bag, place it in 10 times the volume of a polyethylene glycol solution with a weight-to-volume ratio of 15%, and concentrate for 20 hours to obtain a concentrated blend. Vegetarian protein weight volume percentage 20%;

[0067] 4) Place the concentrated blend in a plastic mold and freeze-dry for 48 hours;

[0068] 5) Put the dried blend into water, rinse at 60° for 16 hours, change the water every 4 hours, and change the water 4 times in total to remove polyv...

Embodiment 3

[0072] 1. Purification of silk fibroin

[0073] With embodiment 1.

[0074] 2. Preparation of silk fibroin porous sponge-like scaffold made of polyvinyl alcohol

[0075] 1) Dilute the silk fibroin solution to a concentration of 6% (w / v);

[0076]2) Add 5% (w / v) polyvinyl alcohol solution to the silk fibroin aqueous solution and mix well, the weight ratio of the silk fibroin aqueous solution to the polyvinyl alcohol solution is 1:2;

[0077] 3) Transfer the blend of silk fibroin and polyvinyl alcohol into a dialysis bag, place it in 100 times the volume of a polyethylene glycol solution with a weight-to-volume ratio of 20%, and concentrate for 18 hours to obtain a concentrated blend. Vegetarian protein weight volume percentage 30%;

[0078] 4) Place the concentrated blend in a plastic mold and freeze-dry for 72 hours;

[0079] 5) Put the dried blend into water, rinse at 100° for 8 hours, change the water every 2 hours, and change the water 4 times in total to remove polyv...

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Abstract

The present invention relates to a silk fibroin porous spongy support made from polyvinyl alcohol, and a preparation method and application thereof. The support is made from 60-80 wt% of silk fibroin fiber and 20-40 wt% of polyvinyl alcohol particles; and the support is provided with uniformly distributed micropores, and has a porosity of 85-95%. The preparation method comprises the steps of: 1) extracting silk fibroin from silk to form a silk fibroin aqueous solution; 2) mixing a polyvinyl alcohol solution with the silk fibroin solution; 3) transferring the mixed solution of silk fibroin and polyvinyl alcohol into a dialysis bag, and placing in a polyethylene glycol solution for dialysis concentration; 4) placing the concentrated blend in a plastic mold, and conducting freeze drying; 5) rinsing the dried blend with water, and removing polyvinyl alcohol to form a spongy silk fibroin porous material; and 6) drying the prepared porous support material at room temperature to obtain a finished product. The support is used in tissue engineering, soft tissue repair and drug release.

Description

technical field [0001] The invention relates to a silk fibroin porous scaffold prepared by polyvinyl alcohol, a preparation method and application thereof, and belongs to the technical field of biomaterials. Background technique [0002] Silk fibroin is a natural organic molecule with high molecular weight (about 400KDa) produced in the silkworm. Its content accounts for 70% to 80% of silk and contains 18 kinds of amino acids, 11 of which are essential amino acids for human body; on the other hand, silk fibroin Protein is non-toxic to the human body, safe and reliable, has good biocompatibility, and is suitable for development into functional materials. Therefore, with the in-depth research on its unique amino acid composition and crystal structure and other physical and chemical properties, the application of silk fibroin at home and abroad is actively exploring from the traditional textile field to many fields, and the application of silk fibroin in the field of biomedical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/54A61L27/22A61K47/42
Inventor 张凤莲鲁国明
Owner SUZHOU SIMEITE BIOTECH CO LTD
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