Enzyme-linked immunosorbent assay kit for quantitatively detecting human-serum thyrotrophin receptor antibodies and detecting method
An enzyme-linked immunoassay and thyrotropin technology, which is applied in the field of immunoassays, can solve the problems of low positive detection rate, high price and the like, and achieves the effects of low price, simple method, and easy popularization and application.
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[0031] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.
[0032] Main reagents and raw materials:
[0033] 1. Fusion protein antigen of the carboxy-terminal fragment of the extramembrane region of recombinant hTSHR (prepared by ourselves)
[0034] 2. Carbonate buffer (prepared by yourself)
[0035] 3. Phosphate rinse solution (PBST) (prepared by yourself)
[0036] 4. Blocking solution (prepared by yourself)
[0037] 5. Thyrotropin receptor antibody standard solution (prepared by ourselves)
[0038] 6. Sample diluent (prepared by yourself)
[0039] 7. HRP-labeled goat anti-human IgG secondary antibody (commercially available)
[0040] 8. Chromogenic substrate Tetramethylbenzidine (TMB) (commercially available)
[0041] 9. Reaction termination solution 2M H 2 SO 4 (self-prepared)
[0042] 10. Quality control serum (prepared by yourself)
[0043] 1. Preparation of the fusion protein (TrxFus-hTSHRc) anti...
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